Identification and expression of natriuretic peptide receptor Type-A and-B mRNA in freshwater and seawater rainbow trout

Nankervis, Scott, Powell, Mark, McLeod, Janet and Toop, Tes 2007, Identification and expression of natriuretic peptide receptor Type-A and-B mRNA in freshwater and seawater rainbow trout, Journal of comparative physiology B, vol. 177, no. 2, pp. 259-267, doi: 10.1007/s00360-006-0127-2.

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Title Identification and expression of natriuretic peptide receptor Type-A and-B mRNA in freshwater and seawater rainbow trout
Author(s) Nankervis, Scott
Powell, Mark
McLeod, Janet
Toop, Tes
Journal name Journal of comparative physiology B
Volume number 177
Issue number 2
Start page 259
End page 267
Publisher Springer Verlag
Place of publication Berlin, Germany
Publication date 2007-02
ISSN 0174-1578
1432-136X
Keyword(s) natriuretic peptides
fish osmoregulation
rainbow trout
mRNA expression
natriuretic peptide receptors
Summary Natriuretic peptide receptors mediate the physiological response of  natriuretic peptide hormones. One of the natriuretic peptide receptor types is the particulate guanylyl cyclase receptors, of which there are two identified: NPR-A and NPR-B. In fishes, these have been sequenced and characterized in eels, medaka, and dogfish shark (NPR-B only). The euryhaline rainbow trout provides an opportunity to further pursue examination of the system in teleosts. In this study, partial rainbow trout NPR-A-like and NPR-B-like mRNA sequences were identified via PCR and cloning. The sequence information was used in real-time PCR to examine mRNA expression in a variety of tissues of freshwater rainbow trout and rainbow trout acclimated to 35 parts per thousand seawater for a period of 10 days. In the excretory kidney and posterior intestine, real-time PCR analysis showed greater expression of NPR-B in freshwater fish than in those adapted to seawater; otherwise, there was no difference in the expression of the individual receptors in fresh water or seawater. In general, the expression of the NPR-A and NPR-B type receptors was quite low. These findings indicate that NPR-A and NPR-B mRNA expression is minimally altered under the experimental regime used in this study.
Language eng
DOI 10.1007/s00360-006-0127-2
Field of Research 060602 Animal Physiology - Cell
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2006, Springer-Verlag
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007612

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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