The effect of exercise and insulin on AS160 phosphorylation and 14-3-3 binding capacity in human skeletal muscle

Howlett, Kirsten, Matthews, Alicia, Garnham, Andrew and Sakamoto, Kei 2008, The effect of exercise and insulin on AS160 phosphorylation and 14-3-3 binding capacity in human skeletal muscle, American journal of physiology: endocrinology and metabolism, vol. 294, no. 2, pp. 401-407.

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Title The effect of exercise and insulin on AS160 phosphorylation and 14-3-3 binding capacity in human skeletal muscle
Author(s) Howlett, Kirsten
Matthews, Alicia
Garnham, Andrew
Sakamoto, Kei
Journal name American journal of physiology: endocrinology and metabolism
Volume number 294
Issue number 2
Start page 401
End page 407
Total pages 7
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2008-02
ISSN 0193-1849
1522-1555
Keyword(s) Glucose transport
Type 2 diabetes
Summary AS160 is an Akt substrate of 160 kDa implicated in the regulation of both insulin- and contraction-mediated GLUT4 translocation and glucose uptake. The effects of aerobic exercise and subsequent insulin stimulation on AS160 phosphorylation and the binding capacity of 14-3-3, a novel protein involved in the dissociation of AS160 from GLUT4 vesicles, in human skeletal muscle are unknown. Hyperinsulinemic-euglycemic clamps were performed on seven men at rest and immediately and 3 h after a single bout of cycling exercise. Skeletal muscle biopsies were taken before and after the clamps. The insulin sensitivity index calculated during the final 30 min of the clamp was 8.0 ± 0.8, 9.1 ± 0.5, and 9.2 ± 0.8 for the rest, postexercise, and 3-h postexercise trials, respectively. AS160 phosphorylation increased immediately after exercise and remained elevated 3 h after exercise. In contrast, the 14-3-3 binding capacity of AS160 and phosphorylation of Akt and AMP-activated protein kinase were only increased immediately after exercise. Insulin increased AS160 phosphorylation and 14-3-3 binding capacity and insulin receptor substrate-1 and Akt phosphorylation, but the response to insulin was not enhanced by prior exercise. In conclusion, the 14-3-3 binding capacity of AS160 is increased immediately after acute exercise in human skeletal muscle, but this is not maintained 3 h after exercise completion despite sustained AS160 phosphorylation. Insulin increases AS160 phosphorylation and 14-3-3 binding capacity, but prior exercise does not appear to enhance the response to insulin.
Language eng
Field of Research 111601 Cell Physiology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2007
Copyright notice ©2008, the American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007617

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