Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle

Howlett, Kirsten, Sakamoto, Kei, Garnham, Andrew, Cameron-Smith, David and Hargreaves, Mark 2007, Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle, Diabetes, vol. 56, no. 6, pp. 1608-1614.


Title Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle
Author(s) Howlett, Kirsten
Sakamoto, Kei
Garnham, Andrew
Cameron-Smith, David
Hargreaves, Mark
Journal name Diabetes
Volume number 56
Issue number 6
Start page 1608
End page 1614
Publisher American Diabetes Association
Place of publication New York, N.Y.
Publication date 2007-06
ISSN 0012-1797
1939-327X
Summary A single bout of aerobic exercise can enhance insulin action, but whether a similar effect occurs after resistance exercise is unknown. Hyperinsulinemic-euglycemic clamps were performed on eight male subjects at rest and after a single bout and three repeated bouts of resistance exercise over 7 days. Skeletal muscle biopsies were taken before and after the clamp and immediately after a single exercise bout. Whole-body insulin action measured by glucose infusion rate decreased (P < 0.05) after a single exercise bout, whereas in response to repeated bouts of resistance exercise, the glucose infusion rate was similar to the rest trial. In skeletal muscle, Akt substrate of 160 kDa (AS160) phosphorylation, an Akt substrate implicated in the regulation of GLUT4 translocation, and its interaction with 14-3-3 was decreased (P < 0.05) only after a single exercise bout. Insulin increased (P < 0.05) phosphorylation of AS160 and its interaction with 14-3-3, but the insulin response was not influenced by resistance exercise. Phosphorylation of insulin receptor substrate-1 and Akt were similar to changes in AS160 phosphorylation after exercise and/or insulin. In conclusion, a single bout of resistance exercise impairs whole-body insulin action. Regulation of AS160 and interaction with 14-3-3 in skeletal muscle are influenced by resistance exercise and insulin but do not fully explain the effect of resistance exercise on whole-body insulin action.
Language eng
Field of Research 110306 Endocrinology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2007, American Diabetes Association
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007678

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