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Suppression of bovine viral diarrhea virus replication by small interfering RNA and short hairpin RNA-mediated RNA interference

Lambeth, L., Moore, R., Muralitharan, Morley and Doran, T. 2007, Suppression of bovine viral diarrhea virus replication by small interfering RNA and short hairpin RNA-mediated RNA interference, Veterinary microbiology, vol. 119, no. 2-4, pp. 132-143, doi: 10.1016/j.vetmic.2006.09.008.

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Title Suppression of bovine viral diarrhea virus replication by small interfering RNA and short hairpin RNA-mediated RNA interference
Author(s) Lambeth, L.
Moore, R.
Muralitharan, Morley
Doran, T.
Journal name Veterinary microbiology
Volume number 119
Issue number 2-4
Start page 132
End page 143
Publisher Elsevier Scientific Pub. Co.
Place of publication Amsterdam, Netherlands
Publication date 2007-01
ISSN 0378-1135
1873-2542
Keyword(s) RNA interference
BVDV
Bovine 7SK promoter
Small interfering RNA
Short hairpin RNA
Summary Bovine viral diarrhea virus (BVDV) is a ubiquitous viral pathogen that affects cattle herds’ worldwide causing significant economic loss. The current strategies to control BVDV infection include vaccination (modified-live or killed) and control of virus spread by enhanced biosecurity management, however, the disease remains prevalent. With the discovery of the sequence-specific method of gene silencing known as RNA interference (RNAi), a new era in antiviral therapies has begun. Here we report the efficient inhibition of BVDV replication by small interfering (siRNA) and short hairpin RNA (shRNA)-mediated gene silencing. siRNAs were generated to target the 5′ non-translated (NTR) region and the regions encoding the C, NS4B and NS5A proteins of the BVDV genome. The siRNAs were first validated using an EGFP/BVDV reporter system and were then shown to suppress BVDV-induced cytopathic effects and viral titers in cell culture with surprisingly different activities compared to the reporter system. Efficient viral suppression was then achieved by bovine 7SK-expressed BVDV-specific shRNAs. Overall, our results demonstrated the use of siRNA and shRNA-mediated gene silencing to achieve efficient inhibition of the  replication of this virus in cell culture.
Language eng
DOI 10.1016/j.vetmic.2006.09.008
Field of Research 060499 Genetics not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2006, Elsevier B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007680

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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