Secretion of the glucose-regulated selenoprotein SEPS1 from hepatoma cells

Gao, Yuan, Pagnon, Joanne, Feng, Helen C., Konstantopoulos, Nicky, Jowett, Jeremy B. M. and Walder, Ken 2007, Secretion of the glucose-regulated selenoprotein SEPS1 from hepatoma cells, Biochemical and biophysical research communications, vol. 356, no. 3, pp. 636-641.

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Title Secretion of the glucose-regulated selenoprotein SEPS1 from hepatoma cells
Author(s) Gao, Yuan
Pagnon, Joanne
Feng, Helen C.
Konstantopoulos, Nicky
Jowett, Jeremy B. M.
Walder, Ken
Journal name Biochemical and biophysical research communications
Volume number 356
Issue number 3
Start page 636
End page 641
Publisher Academic Press
Place of publication Orlando, Fla.
Publication date 2007-05-11
ISSN 0006-291X
1090-2104
Keyword(s) selenoprotein
glucose-regulated protein
plasma protein
protein secretion
Summary SEPS1 (also called selenoprotein S, SelS, Tanis or VIMP) is a selenoprotein, localized predominantly in the ER membrane and also on the cell surface. In this report, we demonstrate that SEPS1 protein is also secreted from hepatoma cells but not from five other types of cells examined. The secretion can be abolished by the ER-Golgi transport inhibitor Brefeldin A and by the protein synthesis inhibitor cycloheximide. Using a sandwich ELISA, SEPS1 was detected in the sera of 65 out of 209 human subjects (31.1%, average = 15.7 ± 1.1 ng/mL). Fractionation of human serum indicated that SEPS1 was associated with LDL and possibly with VLDL. The function of plasma SEPS1 is unclear but may be related to lipoprotein metabolism.
Language eng
Field of Research 110199 Medical Biochemistry and Metabolomics not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2007, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007726

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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