Development and application of polymerase chain reaction-based assays for Rathayibacter Toxicus and a Bacteriophage associated with annual ryegrass (Lolium Rigidum) toxicity

Kowalski, M., Cahill, David, Doran, Tim and Colegate, S. 2007, Development and application of polymerase chain reaction-based assays for Rathayibacter Toxicus and a Bacteriophage associated with annual ryegrass (Lolium Rigidum) toxicity, Australian journal of experimental agriculture, vol. 47, pp. 177-183.


Title Development and application of polymerase chain reaction-based assays for Rathayibacter Toxicus and a Bacteriophage associated with annual ryegrass (Lolium Rigidum) toxicity
Author(s) Kowalski, M.
Cahill, David
Doran, Tim
Colegate, S.
Journal name Australian journal of experimental agriculture
Volume number 47
Start page 177
End page 183
Publisher CSIRO Publishing
Place of publication East Melbourne, Vic.
Publication date 2007
ISSN 0816-1089
1836-0939
Keyword(s) 16S rRNA
BLAST search
DNA sequencing
Nested PCR
Summary Annual ryegrass toxicity (ARGT) is responsible for significant stock losses in South Australia and Western Australia. The toxicity is caused by corynetoxins produced by the bacterium Rathayibacter toxicus (with the possible involvement of a bacteriophage), which infects annual ryegrass (Lolium rigidum). Polymerase chain reaction (PCR)-based assays, compatible with an existing enzyme-linked immunosorbent assay for the corynetoxins, have been developed and used to screen L. rigidum for both the presence of R. toxicus and for the bacteriophage isolate NCPPB 3778. The results from analysing bacterially infected galls from toxic grain screenings showed a positive correlation between the presence of the bacterium and corynetoxins but not with the bacteriophage. Analysis of pasture-derived samples of annual ryegrass showed about a 50% correlation of corynetoxins with bacterial presence and about a 5% correlation of phage with the presence of the bacterium. These observations support the potential application of the PCR-based assays in providing a useful, complementary tool in the assessment of the likelihood of pasture and feed to cause ARGT and to enable a better understanding of the complex aetiology of ARGT.
Language eng
Field of Research 070205 Animal Protection (Pests and Pathogens)
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2007, CSIRO
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007757

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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