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Dual mechanisms for nitric oxide control of large arteries in the estuarine crocodile crocodylus porosus

Broughton, Brad and Donald, John 2007, Dual mechanisms for nitric oxide control of large arteries in the estuarine crocodile crocodylus porosus, Journal of experimental biology, vol. 210, no. 1, pp. 129-137, doi: 10.1242/jeb.02620.

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Title Dual mechanisms for nitric oxide control of large arteries in the estuarine crocodile crocodylus porosus
Author(s) Broughton, Brad
Donald, JohnORCID iD for Donald, John orcid.org/0000-0001-5930-2642
Journal name Journal of experimental biology
Volume number 210
Issue number 1
Start page 129
End page 137
Publisher Company of Biologists Ltd.
Place of publication Cambridge, England
Publication date 2007
ISSN 0022-0949
1477-9145
Keyword(s) nitric oxide
nitric oxide synthase
endothelium
nitrergic nerves, ,
blood vessel
vasodilation
Summary n reptiles, accumulating evidence suggests that nitric oxide (NO) induces a potent relaxation in the systemic vasculature. However, very few studies have examined the source from which NO is derived. Therefore, the present study used both anatomical and physiological approaches to establish whether NO-mediated vasodilation is via an endothelial or neural NO pathway in the large arteries of the estuarine crocodile Crocodylus porosus. Specific endothelial nitric oxide synthase (NOS) staining was observed in aortic endothelial cells following nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry and endothelial NOS immunohistochemistry (IHC), suggesting that an endothelial NO pathway is involved in vascular control. This finding was supported by in vitro organ bath physiology, which demonstrated that the relaxation induced by acetylcholine (10-5 mol l-1) was abolished in the presence of the NOS inhibitor, N-omega-nitro-L-arginine (L-NNA; 10-4 mol l-1), the soluble guanylyl cyclase inhibitor, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 10-5 mol l-1), or when the endothelium was removed. Interestingly, evidence for a neural NO pathway was also identified in large arteries of the crocodile. Neural NOS was located in perivascular nerves of the major blood vessels following NADPH-d histochemistry and neural NOS IHC and in isolated aortic rings, L-NNA and ODQ, but not the removal of the endothelium, abolished the relaxation effect of the neural NOS agonist, nicotine (3x10-4 mol l-1). Thus, we conclude that the large arteries of C. porosus are potentially regulated by NO-derived from both endothelial and neural NOS.
Language eng
DOI 10.1242/jeb.02620
Field of Research 060603 Animal Physiology - Systems
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2007, Company of Biologists Ltd.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007814

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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