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Genomic analyses and cloning of novel chicken natriuretic peptide genes reveal new insights into natriuretic peptide evolution

Trajanovska, Sofie, Inoue, Koji, Takei, Yoshio and Donald, John 2007, Genomic analyses and cloning of novel chicken natriuretic peptide genes reveal new insights into natriuretic peptide evolution, Peptides, vol. 28, no. 11, pp. 2155-2163, doi: 10.1016/j.peptides.2007.08.017.

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Title Genomic analyses and cloning of novel chicken natriuretic peptide genes reveal new insights into natriuretic peptide evolution
Author(s) Trajanovska, Sofie
Inoue, Koji
Takei, Yoshio
Donald, JohnORCID iD for Donald, John orcid.org/0000-0001-5930-2642
Journal name Peptides
Volume number 28
Issue number 11
Start page 2155
End page 2163
Publisher Elsevier
Place of publication New York, N.Y.
Publication date 2007-11
ISSN 0196-9781
Keyword(s) chicken
c-type natriuretic peptide
comparative genomics
molecular evolution
synteny
renal natriuretic peptide
Summary The natriuretic peptide (NP) family consists of multiple subtypes in teleosts, including atrial, B-type, ventricular, and C-type NPs (ANP, BNP, VNP, CNP-1–4, respectively), but only ANP, BNP, CNP-3, and CNP-4 have been identified in tetrapods. As part of understanding the molecular evolution of NPs in the tetrapod lineage, we identified NP genes in the chicken genome. Previously, only BNP and CNP-3 have been identified in birds, but we characterized two new chicken NP genes by cDNA cloning, synteny and phylogenetic analyses. One gene is an orthologue of CNP-1, which has only ever been reported in teleostei and bichir. The second gene could not be assigned to a particular NP subtype because of high sequence divergence and was named renal NP (RNP) due to its predominant expression in the kidney. CNP-1 mRNA was only detected in brain, while CNP-3 mRNA was expressed in kidney, heart, and brain. In the developing embryo, BNP and RNP transcripts were most abundant 24 h post-fertilization, while CNP mRNA increased in a stage-dependant manner. Synthetic chicken RNP stimulated an increase in cGMP production above basal level in chicken kidney membrane preparations and caused a potent dose-dependant vasodilation of pre-constricted dorsal aortic rings. From conserved chromosomal synteny, we propose that the CNP-4 and ANP genes have been lost in chicken, and that RNP may have evolved from a VNP-like gene. Furthermore, we have demonstrated for the first time that CNP-1 is retained in the tetrapod lineage.
Language eng
DOI 10.1016/j.peptides.2007.08.017
Field of Research 060603 Animal Physiology - Systems
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2007, Elsevier Inc.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007816

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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