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Environmental factors affecting transcription of the human L1 retrotransposon. I. Steroid hormone-like agents

Morales, José F., Snow, Elizabeth T. and Murnane, John P. 2002, Environmental factors affecting transcription of the human L1 retrotransposon. I. Steroid hormone-like agents, Mutagenesis, vol. 17, no. 3, pp. 193-200.

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Title Environmental factors affecting transcription of the human L1 retrotransposon. I. Steroid hormone-like agents
Author(s) Morales, José F.
Snow, Elizabeth T.
Murnane, John P.
Journal name Mutagenesis
Volume number 17
Issue number 3
Start page 193
End page 200
Total pages 8 p.
Publisher Oxfod University Press
Place of publication Oxford, England
Publication date 2002
ISSN 0267-8357
1464-3804
Summary The L1 retrotransposon has significantly shaped the structure of the human genome. At least 30% of human genome sequence can be attributed to L1 reverse transcriptase activity. There are 105 copies of the human L1 retrotransposon, L1Hs, most of which are defective, although ~8–9x103 are full length. L1Hs elements transpose through an RNA intermediate and transcription is thought to be the rate limiting step in retrotransposition. Because transcription of retrotransposons in a variety of organisms has been shown to respond to environmental stimuli, we investigated the influence of various agents on transcription from two different L1Hs promoters. The activity of the L1Hs promoters was analyzed by transfecting L1Hs-expressing cell lines with plasmids containing the L1Hs promoters fused to the LacZ reporter gene and monitoring expression with a ß-galactosidase assay. Small increases in ß-galactosidase activity were observed with both L1Hs promoters after treatment with serum, testosterone, dihydrotestosterone and organochloride pesticides, indicating that these agents can influence L1Hs transcription.
Language eng
Field of Research 060405 Gene Expression (incl Microarray and other genome-wide approaches)
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30008546

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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