Interaction of exercise and diet on GLUT-4 protein and gene expression in type I and type II rat skeletal muscle

Lee, J., Bruce, C., Tunstall, R., Cameron-Smith, David, Hugel, H. and Hawley, J. 2002, Interaction of exercise and diet on GLUT-4 protein and gene expression in type I and type II rat skeletal muscle, Acta physiologica Scandinavica, vol. 175, no. 1, pp. 37-44.

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Title Interaction of exercise and diet on GLUT-4 protein and gene expression in type I and type II rat skeletal muscle
Author(s) Lee, J.
Bruce, C.
Tunstall, R.
Cameron-Smith, David
Hugel, H.
Hawley, J.
Journal name Acta physiologica Scandinavica
Volume number 175
Issue number 1
Start page 37
End page 44
Publisher Blackwell Publishing Limited
Place of publication Oxford, England
Publication date 2002-05
ISSN 0001-6772
1365-201X
Keyword(s) exercise-training
high-fat diet
insulin-resistance
muscle triacylglycerol
Summary We determined the interaction of exercise and diet on glucose transporter (GLUT-4) protein and mRNA expression in type I (soleus) and type II [extensor digitorum longus (EDL)] skeletal muscle. Forty-eight Sprague Dawley rats were randomly assigned to one of two dietary conditions: high-fat (FAT, n =24) or high-carbohydrate (CHO, n =24). Animals in each dietary condition were allocated to one of two groups: control (NT, n =8) or a group that performed 8 weeks of treadmill running (4 sessions week<sup>–1</sup> of 1000 m @ 28 m min<sup>–1</sup> , RUN, n =16). Eight trained rats were killed after their final exercise bout for determination of GLUT-4 protein and mRNA expression: the remainder were killed 48 h after their last session for measurement of muscle glycogen and triacylglycerol concentration. GLUT-4 protein expression in NT rats was similar in both muscles after 8 weeks of either diet. However, there was a main effect of training such that GLUT-4 protein was increased in the soleus of rats fed with either diet (P < 0.05) and in the EDL in animals fed with CHO (P < 0.05). There was a significant diet–training interaction on GLUT-4 mRNA, such that expression was increased in both the soleus (100% ↑P < 0.05) and EDL (142% ↑P < 0.01) in CHO-fed animals. Trained rats fed with FAT decreased mRNA expression in the EDL (↓ 45%, P < 0.05) but not the soleus (↓ 14%, NS). We conclude that exercise training in CHO-fed rats increased both GLUT-4 protein and mRNA expression in type I and type II skeletal muscle. Despite lower GLUT-4 mRNA in muscles from fat-fed animals, exercise-induced increases in GLUT-4 protein were largely preserved, suggesting that control of GLUT-4 protein and gene expression are modified independently by exercise and diet.
Language eng
Field of Research 110602 Exercise Physiology
Socio Economic Objective 970111
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2002, Scandinavian Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30008556

Document type: Journal Article
Collection: School of Health Sciences
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