Regulation of metabolic genes in human skeletal muscle by short-term exercise and diet manipulation

Arkinstall, Melissa J., Tunstall, Rebecca J., Cameron-Smith, David and Hawley, John A. 2004, Regulation of metabolic genes in human skeletal muscle by short-term exercise and diet manipulation, American journal of physiology. Endocrinology and metabolism, vol. 287, pp. 25-31.

Attached Files
Name Description MIMEType Size Downloads

Title Regulation of metabolic genes in human skeletal muscle by short-term exercise and diet manipulation
Author(s) Arkinstall, Melissa J.
Tunstall, Rebecca J.
Cameron-Smith, David
Hawley, John A.
Journal name American journal of physiology. Endocrinology and metabolism
Volume number 287
Start page 25
End page 31
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2004-02-03
ISSN 0193-1849
1522-1555
Keyword(s) messenger ribonucleic acid
exercise-diet gene interaction
Summary Changes in dietary macronutrient intake alter muscle and blood substrate availability and are important for regulating gene expression. However, few studies have examined the effects of diet manipulation on gene expression in human skeletal muscle. The aim of this study was to quantify the extent to which altering substrate availability impacts on subsequent mRNA abundance of a subset of carbohydrate (CHO)- and fat-related genes. Seven subjects consumed either a low- (LOW; 0.7 g/kg body mass CHO) or high- (HIGH; 10 g/kg body mass CHO) CHO diet for 48 h after performing an exhaustive exercise bout to deplete muscle glycogen stores. After intervention, resting muscle and blood samples were taken. Muscle was analyzed for the gene abundances of GLUT4, glycogenin, pyruvate dehydrogenase kinase-4 (PDK-4), fatty acid translocase (FAT/CD36), carnitine palmitoyltransferase I (CPT I), hormone-sensitive lipase (HSL), β-hydroxyacyl-CoA dehydrogenase (΄β-HAD), and uncoupling binding protein-3 (UCP3), and blood samples for glucose, insulin, and free fatty acid (FFA) concentrations. Glycogen-depleting exercise and HIGH-CHO resulted in a 300% increase in muscle glycogen content (P < 0.001) relative to the LOW-CHO condition. FFA concentrations were twofold higher after LOW- vs. HIGH-CHO (P < 0.05). The exercise-diet manipulation exerted a significant effect on transcription of all carbohydrate-related genes, with an increase in GLUT4 and glycogenin mRNA abundance and a reduction in PDK-4 transcription after HIGH-CHO (all P < 0.05). FAT/CD36 (P < 0.05) and UCP3 (P < 0.01) gene transcriptions were increased following LOW-CHO. We conclude that 1) there was a rapid capacity for a short-term exercise and diet intervention to exert coordinated changes in the mRNA transcription of metabolic related genes, and 2) genes involved in glucose regulation are increased following a high-carbohydrate diet.
Language eng
Field of Research 110602 Exercise Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2004, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30008697

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 35 times in TR Web of Science
Scopus Citation Count Cited 43 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 442 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Mon, 13 Oct 2008, 15:39:24 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.