Effects of aerobic training on pyruvate dehydrogenase and pyruvate dehydrogenase kinase in human skeletal muscle

Leblanc, Paul, Peters, Sandra, Tunstall, Rebecca, Cameron-Smith, David and Heigenhauser, George 2004, Effects of aerobic training on pyruvate dehydrogenase and pyruvate dehydrogenase kinase in human skeletal muscle, Journal of physiology, vol. 557, no. 2, pp. 559-570.

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Title Effects of aerobic training on pyruvate dehydrogenase and pyruvate dehydrogenase kinase in human skeletal muscle
Author(s) Leblanc, Paul
Peters, Sandra
Tunstall, Rebecca
Cameron-Smith, David
Heigenhauser, George
Journal name Journal of physiology
Volume number 557
Issue number 2
Start page 559
End page 570
Publisher Cambridge University Press
Place of publication London, England
Publication date 2004-06
ISSN 0022-3751
1469-7793
Summary This study examined the effects of short- and long-term aerobic training on the stable up-regulation of pyruvate dehydrogenase (PDH) and PDH kinase (PDK) in human skeletal muscle. We hypothesized that 8 weeks, but not 1 week, of aerobic training would increase total PDH (PDHt) and PDK activities compared to pretraining, and this would be detectable at the level of gene transcription (mRNA) and/or gene translation (protein). Resting muscle biopsies were taken before and after 1 and 8 weeks of aerobic cycle exercise training. PDHt and PDK activities, and their respective protein and mRNA expression, did not differ after 1 week of aerobic training. PDHt activity increased 31% after 8 weeks and this may be partially due to a 1.3-fold increase in PDH-E1α protein expression. PDK activity approximately doubled after 8 weeks of aerobic training and this was attributed to a 1.3-fold increase in PDK2 isoform protein expression. Similar to 1 week, no changes were observed at the mRNA level after 8 weeks of training. These findings  suggest that aerobically trained human skeletal muscle has an increased maximal capacity to utilize carbohydrates, evident by increased PDHt, but increased metabolic control sensitivity to pyruvate through increased contribution of PDK2 to total PDK activity.
Language eng
Field of Research 110602 Exercise Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2004, The Physiological Society.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30008700

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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