You are not logged in.

Immunolocalization of Na+/K+-ATPase, carbonic anhydrase II, and vacuolar H+-ATPase in the gills of freshwater adult lampreys, Geotria australis

Choe, Keith P., O`Brien, Siobhan, Evans, David H., Toop, Tes and Edwards, Susan L. 2004, Immunolocalization of Na+/K+-ATPase, carbonic anhydrase II, and vacuolar H+-ATPase in the gills of freshwater adult lampreys, Geotria australis, Journal of experimental zoology part a: comparative experimental biology, vol. 301A, no. 8, pp. 654-665, doi: 10.1002/jez.a.60.

Attached Files
Name Description MIMEType Size Downloads

Title Immunolocalization of Na+/K+-ATPase, carbonic anhydrase II, and vacuolar H+-ATPase in the gills of freshwater adult lampreys, Geotria australis
Formatted title Immunolocalization of Na+/K+-ATPase, carbonic anhydrase II, and vacuolar H+-ATPase in the gills of freshwater adult lampreys, Geotria australis
Author(s) Choe, Keith P.
O`Brien, Siobhan
Evans, David H.
Toop, Tes
Edwards, Susan L.
Journal name Journal of experimental zoology part a: comparative experimental biology
Volume number 301A
Issue number 8
Start page 654
End page 665
Publisher John Wiley & Sons
Place of publication New York, N.Y.
Publication date 2004-08-01
ISSN 1932-5223
1932-5231
Summary As adults, anadromous lampreys migrate from seawater into freshwater rivers, where they require branchial ion (NaCl) absorption for osmoregulation. In teleosts and elasmobranchs, pharmological, immunohistochemical, and molecular data support roles for Na+/K+-ATPase (NPPase), carbonic anhydrase II (CAII), and vacuolar H+-ATPase (V-ATPase) in two different models of branchial ion absorption. To our knowledge, these transport-related proteins have not been studied in adult freshwater lampreys, and therefore it is not known if they are expressed, or have similar functions, in lampreys. The purpose of this study was to localize NPPase, CAII, and V-ATPase in the gills of adult freshwater lampreys and determine if any of these transport-related proteins are expressed in the same cells. Heterologous antibodies were used to localize the three proteins in gill tissue from pouched lamprey (Geotria australis). Immunoreactivity (IR) for all three proteins occurred between, and at the base of, lamellae in cells that match previous descriptions of mitochondrion-rich-cells (MRCs). NPPase-IR was always on the basolateral side of cells that did not stain for CAII or V-ATPase. In contrast, CAII-IR was always on the apical side of cells that also contained diffuse V-ATPase-IR. Therefore, we have identified two types of MRC in adult freshwater lamprey gills based on immunohistochemical staining for three transport proteins. A model of ion transport, based on our results, is proposed for adult freshwater lampreys. 
Language eng
DOI 10.1002/jez.a.60
Field of Research 060899 Zoology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2006, Wiley-Liss, Inc
Persistent URL http://hdl.handle.net/10536/DRO/DU:30008770

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 20 times in TR Web of Science
Scopus Citation Count Cited 21 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 505 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Mon, 13 Oct 2008, 15:41:29 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.