The effects of exercise and adipose tissue lipolysis on plasma adiponectin concentration and adiponectin receptor expression in human skeletal muscle

Punyadeera, Chamindie, Zorenc, Antone, Koopman, René, McAinch, Andrew, Smit, Egbert, Manders, Ralph, Keizer, Hans, Cameron-Smith, David and van Loon, Luc 2005, The effects of exercise and adipose tissue lipolysis on plasma adiponectin concentration and adiponectin receptor expression in human skeletal muscle, European journal of endocrinology, vol. 152, no. 3, pp. 427-436.

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Title The effects of exercise and adipose tissue lipolysis on plasma adiponectin concentration and adiponectin receptor expression in human skeletal muscle
Author(s) Punyadeera, Chamindie
Zorenc, Antone
Koopman, René
McAinch, Andrew
Smit, Egbert
Manders, Ralph
Keizer, Hans
Cameron-Smith, David
van Loon, Luc
Journal name European journal of endocrinology
Volume number 152
Issue number 3
Start page 427
End page 436
Publisher Scandinavian University Press
Place of publication Oslo, Norway
Publication date 2005
ISSN 0804-4635
1479-683X
Summary Objective: It has been suggested that adiponectin regulates plasma free fatty acid (FFA) clearance by stimulating FFA uptake and/or oxidation in muscle. We aimed to determine changes in plasma adiponectin concentration and adiponectin receptor 1 and 2 mRNA expression in skeletal muscle during and after prolonged exercise under normal, fasting conditions (high FFA trial; HFA) and following pharmacological inhibition of adipose tissue lipolysis (low FFA trial; LFA). Furthermore, we aimed to detect and locate adiponectin in skeletal muscle tissue. Methods: Ten subjects performed two exercise trials (120 min at 50% VO2max). Indirect calorimetry was used to determine total fat oxidation rate. Plasma samples were collected at rest, during exercise and during post-exercise recovery to determine adiponectin, FFA and glycerol concentrations. Muscle biopsies were taken to determine adiponectin protein and adiponectin receptor 1 and 2 mRNA expression and to localise intramyocellular adiponectin. Results: Basal plasma adiponectin concentrations averaged 6.57±0.7 and 6.63±0.8 mg/l in the HFA and LFA trials respectively, and did not change significantly during or after exercise. In the LFA trial, plasma FFA concentrations and total fat oxidation rates were substantially reduced. However, plasma adiponectin and muscle adiponectin receptor 1 and 2 mRNA expression did not differ between trials. Immunohistochemical staining of muscle cross-sections showed the presence of adiponectin in the sarcolemma of individual muscle fibres and within the interfibrillar arterioles. Conclusion: Plasma adiponectin concentrations and adiponectin receptor 1 and 2 mRNA expression in muscle are not acutely regulated by changes in adipose tissue lipolysis and/or plasma FFA concentrations. Adiponectin is abundantly expressed in muscle, and, for the first time, it has been shown to be present in/on the sarcolemma of individual muscle fibres.
Language eng
Field of Research 110306 Endocrinology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2005, Society of the European Journal of Endocrinology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30008881

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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