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Mitochondrial DNA diversity of broodstock of two indigenous mahseer species, Tor tambroides and T. douronensis (Cyprinidae) cultured in Sarawak, Malaysia

Nguyen, Thuy, Ingram, Brett, Sungan, Stephen, Gooley, Geoff, Sim, Sih Yang, Tinggi, David and De Silva, Sena 2006, Mitochondrial DNA diversity of broodstock of two indigenous mahseer species, Tor tambroides and T. douronensis (Cyprinidae) cultured in Sarawak, Malaysia, Aquaculture, vol. 253, no. 1-4, pp. 259-269, doi: 10.1016/j.aquaculture.2005.09.014.

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Title Mitochondrial DNA diversity of broodstock of two indigenous mahseer species, Tor tambroides and T. douronensis (Cyprinidae) cultured in Sarawak, Malaysia
Formatted title Mitochondrial DNA diversity of broodstock of two indigenous mahseer species, Tor tambroides and T. douronensis (Cyprinidae) cultured in Sarawak, Malaysia
Author(s) Nguyen, Thuy
Ingram, Brett
Sungan, Stephen
Gooley, Geoff
Sim, Sih Yang
Tinggi, David
De Silva, SenaORCID iD for De Silva, Sena orcid.org/0000-0001-9381-2136
Journal name Aquaculture
Volume number 253
Issue number 1-4
Start page 259
End page 269
Publisher Elsevier Scientific Pub. Co.
Place of publication Amsterdam, Netherlands
Publication date 2006-03
ISSN 0044-8486
1873-5622
Keyword(s) T. tambroides and ; ; ; ; ;
T. douronensis
mahseer
broodstock
MtDNA
16S tRNA
conservation
Summary Tor tambroides and T. douronensis, locally referred to as empurau and semah, respectively, are high valued mahseer species, indigenous to Sarawak, East Malaysia, with an aquaculture potential and of conservational value. Direct sequencing of mitochondrial DNA (mtDNA) 16S rRNA gene region (542 bp) was used to investigate genetic variation of T. tambroides and T. douronensis broodstock collected from different geographic locations in Sarawak and maintained at the Indigenous Fish Research and Production Center (IFRPC), Tarat, Sarawak, Malaysia. A total of 11 unique haplotypes were identified, of which six were detected in T. tambroides, and five in T. douronensis. Overall, nucleotide diversity (π) was low, ranging from 0.000 to 0.006, and haplotype diversity (h) ranged from 0.000 to 0.599. Although the analysis failed to detect genetic variation amongst populations of T. tambroides (significant pairwise FST was found for only one test, but pairwise haplotype frequencies were not statistically significant), substantial inter-population divergence among T. douronensis was recognised, especially those originating from different river systems (pairwise FST = 0.754 to 1.000, P < 0.05). Fixed haplotype differences were found in one population of T. douronensis. Average nucleotide divergence between T. tambroides and T. douronensis was 0.018, similar to the amount recognised between T. tambroides and the outgroup T. khudree (0.017). In addition, phylogenetic analysis revealed that the T. douronensis mtDNA consisted of two highly divergent clusters (0.020), one of which is more closely related to T. tambroides rather than with the other group of haplotypes of the conspecifics. The findings from the present study have important implications for aquaculture, management and conservation of these two species. The data also raise some concerns regarding the taxonomic status of T. douronensis, which needs to be addressed.
Language eng
DOI 10.1016/j.aquaculture.2005.09.014
Field of Research 070401 Aquaculture
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2005, Elsevier B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30008968

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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