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Trapping of a spiral-like intermediate of the bacterial cytokinetic protein FtsZ

Michie, Katherine A., Monahan, Leigh G., Beech, Peter and Harry, Elizabeth J. 2006, Trapping of a spiral-like intermediate of the bacterial cytokinetic protein FtsZ, Journal of bacteriology, vol. 188, no. 5, pp. 1680-1690, doi: 10.1128/JB.188.5.1680-1690.2006.

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Title Trapping of a spiral-like intermediate of the bacterial cytokinetic protein FtsZ
Author(s) Michie, Katherine A.
Monahan, Leigh G.
Beech, Peter
Harry, Elizabeth J.
Journal name Journal of bacteriology
Volume number 188
Issue number 5
Start page 1680
End page 1690
Publisher American Society for Microbiology
Place of publication Washington, D.C.
Publication date 2006-03
ISSN 0021-9193
Summary The earliest stage in bacterial cell division is the formation of a ring, composed of the tubulin-like protein FtsZ, at the division site. Tight spatial and temporal regulation of Z-ring formation is required to ensure that division occurs precisely at midcell between two replicated chromosomes. However, the mechanism of Z-ring formation and its regulation in vivo remain unresolved. Here we identify the defect of an interesting temperature-sensitive ftsZ mutant (ts1) of Bacillus subtilis. At the nonpermissive temperature, the mutant protein, FtsZ(Ts1), assembles into spiral-like structures between chromosomes. When shifted back down to the permissive temperature, functional Z rings form and division resumes. Our observations support a model in which Z-ring formation at the division site arises from reorganization of a long cytoskeletal spiral form of FtsZ and suggest that the FtsZ(Ts1) protein is captured as a shorter spiral-forming intermediate that is unable to complete this reorganization step. The ts1 mutant is likely to be very valuable in revealing how FtsZ assembles into a ring and how this occurs precisely at the division site.

Language eng
DOI 10.1128/JB.188.5.1680-1690.2006
Field of Research 060501 Bacteriology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2006, American Society for Microbiology
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30009008

Document type: Journal Article
Collections: School of Life and Environmental Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.