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Effect of Glucocorticoid pretreatment on oxidative liver injury and survival in jaundiced rats with Endotoxing Cholangitis

Lee, Chi Wei, Chaung, Jin Haur, Wang, Pei Wen, Chang, Nyuk Kong, Wang, Hsiu Chuan, Huang, Chao Cheng, Tiao, Mao Meng and Lo, Sing Kai 2006, Effect of Glucocorticoid pretreatment on oxidative liver injury and survival in jaundiced rats with Endotoxing Cholangitis, World journal of surgery, vol. 30, no. 12, pp. 2217-2226.

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Title Effect of Glucocorticoid pretreatment on oxidative liver injury and survival in jaundiced rats with Endotoxing Cholangitis
Author(s) Lee, Chi Wei
Chaung, Jin Haur
Wang, Pei Wen
Chang, Nyuk Kong
Wang, Hsiu Chuan
Huang, Chao Cheng
Tiao, Mao Meng
Lo, Sing Kai
Journal name World journal of surgery
Volume number 30
Issue number 12
Start page 2217
End page 2226
Publisher Springer Verlag
Place of publication New York, N.Y.
Publication date 2006-12
ISSN 0364-2313
1432-2323
Summary Introduction: Biliary tract infection is associated with high mortality. This study investigated the effect of glucocorticoid pretreatment on lipopolysaccharide (LPS)-induced cholangitis. Methods: Rats undergoing either sham operation or ligation of the extrahepatic bile duct (BDL) for 2 weeks were randomly assigned to receive intravenous injections of dexamethasone (DX) or normal saline (NS) prior to infusing LPS into the biliary tract. The plasma levels of tumor necrosis factor-α (TNFα), chemokines monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-2 (MIP-2) as well as liver mRNA expression of MCP-1 and MIP-2 were determined. Infiltration of monocytes, Kupffer cells, and neutrophils in rat liver were studied with immunohistochemistry. Oxidative liver injury was measured by the malondialdehyde (MDA) content. Results: Dexamethasone pretreatment resulted in significantly decreased plasma levels of TNFα at 1 hour, MCP-1 and MIP-2 at 2 and 3 hours, and decreased liver MCP-1 mRNA expression at 3 hours following LPS infusion in BDL-DX rats than in BDL-NS rats. The number of inflammatory cells in the liver was significantly different between sham- and BDL-treated rats but was not affected by DX pretreatment. Pretreatment with DX resulted in significantly decreased liver MDA contents in the BDL-DX group than that in the BDL-NS group. Jaundiced rats pretreated with 5 mg DX prior to infusion of 1 g of LPS were 6.8 times more likely to survive than those that were not pretreated. Conclusions: Pretreatment of jaundiced, LPS-treated rats with a  supraphysiological dose of dexamethasone may rescue their lives by suppression of chemokine expression and alleviation of oxidative liver injury.
Language eng
Field of Research 110307 Gastroenterology and Hepatology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2006, Socie´te´ Internationale de Chirurgie
Persistent URL http://hdl.handle.net/10536/DRO/DU:30009036

Document type: Journal Article
Collection: School of Health and Social Development
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Created: Mon, 13 Oct 2008, 15:49:15 EST