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Expression of uncoupling protein-3 subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its moduclation by fasting

Jimenez, Maria, Yvon, Cedric, Lehr, Lorenz, Leger, Bertrand, Keller, Patrick, Russell, Aaron, Kuhne, Francoise, Flandin, Pierre, Giacobino, Jean-Paul and Muzzin, Patrick 2002, Expression of uncoupling protein-3 subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its moduclation by fasting, The FEBS journal, vol. 269, no. 12, pp. 2878-2884, doi: 10.1046/j.1432-1033.2002.02953.x.

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Title Expression of uncoupling protein-3 subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its moduclation by fasting
Author(s) Jimenez, Maria
Yvon, Cedric
Lehr, Lorenz
Leger, Bertrand
Keller, Patrick
Russell, Aaron
Kuhne, Francoise
Flandin, Pierre
Giacobino, Jean-Paul
Muzzin, Patrick
Journal name The FEBS journal
Volume number 269
Issue number 12
Start page 2878
End page 2884
Publisher Blackwell Publishing Ltd
Place of publication Oxford, England
Publication date 2002-06
ISSN 1742-464x
1742-4658
Keyword(s) fasting
intermyofibrillar mitochondria
muscle type
subsacorlemmal
uncoupling protein-3 (UCP3)
Summary Uncoupling protein-3 (UCP3) is a mitochondrial inner-membrane protein abundantly expressed in rodent and human skeletal muscle which may be involved in energy dissipation. Many studies have been performed on the metabolic regulation of UCP3 mRNA level, but little is known about UCP3 expression at the protein level. Two populations of mitochondria have been described in skeletal muscle, subsarcolemmal (SS) and intermyofibrillar (IMF), which differ in their intracellular localization and possibly also their metabolic role. To examine if UCP3 is differentially expressed in these two populations and in different mouse muscle types, we developed a new protocol for isolation of SS and IMF mitochondria and carefully validated a new UCP3 antibody. The data show that the density of UCP3 is higher in the mitochondria of glycolytic muscles (tibialis anterior and gastrocnemius) than in those of oxidative muscle (soleus). They also show that SS mitochondria contain more UCP3 per mg of protein than IMF mitochondria. Taken together, these results suggest that oxidative muscle and the mitochondria most closely associated with myofibrils are most efficient at producing ATP. We then determined the effect of a 24-h fast, which greatly increases UCP3 mRNA (16.4-fold) in muscle, on UCP3 protein expression in gastrocnemius mitochondria. We found that fasting moderately increases (1.5-fold) or does not change UCP3 protein in gastrocnemius SS or IMF mitochondria, respectively. These results show that modulation of UCP3 expression at the mRNA level does not necessarily result in similar changes at the protein level and indicate that UCP3 density in SS and IMF mitochondria can be differently affected by metabolic changes.
Language eng
DOI 10.1046/j.1432-1033.2002.02953.x
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2002, FEBS
Persistent URL http://hdl.handle.net/10536/DRO/DU:30009116

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