Early induction of chalpains in rotenone-mediated neuronal apoptosis

Chen, Minghui Jessica, Yap, Yann Wan, Choy, Meng Shyan, Koh, Chor Hui Vivien, Seet, Sze Jee, Duan, Wei, Whiteman, Matthew and Cheung, Nam Sang 2006, Early induction of chalpains in rotenone-mediated neuronal apoptosis, Neuroscience letters, vol. 397, no. 1-2, pp. 69-73.

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Title Early induction of chalpains in rotenone-mediated neuronal apoptosis
Author(s) Chen, Minghui Jessica
Yap, Yann Wan
Choy, Meng Shyan
Koh, Chor Hui Vivien
Seet, Sze Jee
Duan, Wei
Whiteman, Matthew
Cheung, Nam Sang
Journal name Neuroscience letters
Volume number 397
Issue number 1-2
Start page 69
End page 73
Publisher Elsvier Ireland Ltd
Place of publication Limerick, Ireland
Publication date 2006-04
ISSN 0304-3940
1872-7972
Keyword(s) Parkinson's disease
rotenone
apoptosis
caspases
calpains
cell cycle re-entry
Summary Rotenone is an inhibitor of mitochondrial complex I that produces a model of Parkinson's disease (PD), where neurons undergo apoptosis by caspase-dependent and/or caspase-independent pathways. Inhibition of calpains has recently been shown to attenuate neuronal apoptosis. This study aims to establish for the first time, the time-point of calpain activation with respect to the caspase activation and the possibility of cell cycle re-entry in rotenone-mediated cell death. Immunoblot results revealed calpain activation occurred at 5, 10 h prior to caspase-3 activation (at 15 h), suggesting calpain activation was an earlier cellular event compared to caspase activation in the rotenone-mediated apoptosis. In addition, an upregulation of phospho-p53 was observed at 21 h. However, no expression or upregulation of cell cycle regulatory proteins including cdc25a, cyclin-D1 and cyclin-D3 were observed, strongly suggesting that cell cycle re-entry did not occur. These findings provide new insights into the differential patterns of calpain and caspase activation that result from rotenone poisoning and which may be relevant to the therapeutic management of PD.
Language eng
Field of Research 110902 Cellular Nervous System
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2005, Elsevier Ireland Ltd.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30009162

Document type: Journal Article
Collection: School of Medicine
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