A study of the effects of flux density and frequency on Pulsed electromagnetic field on Neurite outgrowth in PC12 cells.

Zhang, Yang, Ding, Jun and Duan, Wei 2006, A study of the effects of flux density and frequency on Pulsed electromagnetic field on Neurite outgrowth in PC12 cells., Journal of biological physics: an intrnational journal for the formulation and application of physical and mathematical models in the biological sciences, vol. 32, no. 10, pp. 1-9.

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Title A study of the effects of flux density and frequency on Pulsed electromagnetic field on Neurite outgrowth in PC12 cells.
Author(s) Zhang, Yang
Ding, Jun
Duan, Wei
Journal name Journal of biological physics: an intrnational journal for the formulation and application of physical and mathematical models in the biological sciences
Volume number 32
Issue number 10
Start page 1
End page 9
Publisher Springer Netherlands
Place of publication Amsterdam, The Netherlands
Publication date 2006-01
ISSN 0092-0606
1573-0689
Keyword(s) nerve growth factor
neurite outgrowth
PC12 cells
pulse frequency
pulsed electromagnetic field
Summary The aim of this study was to investigate the influence of pulsed electromagnetic fields with various flux densities and frequencies on neurite outgrowth in PC12 rat pheochromocytoma cells. We have studied the percentage of neurite-bearing cells, average length of neurites and directivity of neurite outgrowth in PC12 cells cultured for 96 hours in the presence of nerve growth factor (NGF). PC12 cells were exposed to 50 Hz pulsed electromagnetic fields with a flux density of 1.37 mT, 0.19 mT and 0.016 mT respectively. The field was generated through a Helmholtz coil pair housed in one incubator and the control samples were placed in another identical incubator. It was found that exposure to both a relatively high flux density (1.37 mT) and a medium flux density (0.19 mT) inhibited the percentage of neurite-bearing cells and promoted neurite length significantly. Exposure to high flux density (1.37 mT) also resulted in nearly 20% enhancement of neurite directivity along the field direction. However, exposure to low flux density field (0.016 mT) had no detectable effect on neurite outgrowth. We also studied the effect of frequency at the constant flux density of 1.37 mT. In the range from 1 ~ 100 Hz, only 50 and 70 Hz pulse frequencies had significant effects on neurite outgrowth. Our study has shown that neurite outgrowth in PC12 cells is sensitive to flux density and frequency of pulsed electromagnetic field.
Language eng
Field of Research 110902 Cellular Nervous System
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2006, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30009164

Document type: Journal Article
Collection: School of Medicine
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