Proteomic analysis of macrophage differentiation

Csar, Xavier F., Wilson, Nicholas J., McMahon, Kerrie-Ann, Marks, Denese C., Beecroft, Tina L., Ward, Alister, Whitty, Genevieve A., Kanangasundarum, Varuni and Hamilton, John A. 2001, Proteomic analysis of macrophage differentiation, Journal of Biological Chemistry, vol. 276, no. 28, pp. 26211-26217.

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Title Proteomic analysis of macrophage differentiation
Author(s) Csar, Xavier F.
Wilson, Nicholas J.
McMahon, Kerrie-Ann
Marks, Denese C.
Beecroft, Tina L.
Ward, Alister
Whitty, Genevieve A.
Kanangasundarum, Varuni
Hamilton, John A.
Journal name Journal of Biological Chemistry
Volume number 276
Issue number 28
Start page 26211
End page 26217
Publisher American Society for Biochemistry and Molecular Biology
Place of publication United States
Publication date 2001-07-13
ISSN 0021-9258
1083-351X
Notes Macrophage colony stimulating factor (M-CSF or CSF-1) acts to regulate the development and function of cells of the macrophage lineage. Murine myeloid FDC-P1 cells transfected with the CSF-1 receptor (FD/WT) adopt a macrophage-like morphology when cultured in CSF-1. This process is abrogated in FDC-P1 cells transfected with the CSF-1 receptor with a tyrosine to phenyalanine substitution at position 807 (FD/807), suggesting that a molecular interaction critical to differentiation signaling is lost (Bourette, R. P., Myles, G. M., Carlberg, K., Chen, A. R., and Rohrschneider, L. R. (1995) Cell Growth Differ. 6, 631-645). A detailed examination of lysates of CSF-1-treated FD/807 cells by two-dimensional SDS-polyacrylamide gel electrophoresis (PAGE) revealed a number of proteins whose degree of tyrosine phosphorylation was modulated by the Y807F mutation. Included in this category were three phosphorylated proteins that co-migrated with p46/52Shc. Immunoprecipitation, Western blotting, and in vitro binding studies suggest that they are indeed p46/52Shc. A key regulator of differentiation in a number of cell systems, ERK was observed to exhibit an activity that correlated with the relative degree of differentiation induced by CSF-1 in the two cell types. Transfection of cells with a non-tyrosine-phosphorylatable form of p46/52Shc prevented the normally observed CSF-1-mediated macrophage differentiation as determined by adoption of macrophage-like morphology and expression of the monocyte/macrophage lineage cell surface marker, Mac-1. These results are the first to suggest that p46/52Shc may play a role in CSF-1-induced macrophage differentiation. Additionally, a number of proteins were identified by two-dimensional SDS-PAGE whose degree of tyrosine phosphorylation is also modulated by the Y807F substitution. This group of molecules may contain novel signaling molecules important in macrophage differentiation.
Language eng
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2001 by the American Society for Biochemistry and Molecular Biology.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30009175

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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