C/EBPÁ and G-CSF receptor signals cooperate to induce the myeloperoxidase and neutrophil elastase genes

Wang, W., Wang, X., Ward, Alister, Touw, I. and Friedman, A. 2001, C/EBPÁ and G-CSF receptor signals cooperate to induce the myeloperoxidase and neutrophil elastase genes, Leukemia, vol. 15, no. 5, pp. 779-786.

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Title C/EBPÁ and G-CSF receptor signals cooperate to induce the myeloperoxidase and neutrophil elastase genes
Author(s) Wang, W.
Wang, X.
Ward, Alister
Touw, I.
Friedman, A.
Journal name Leukemia
Volume number 15
Issue number 5
Start page 779
End page 786
Publisher Nature Publishing Group
Place of publication London, England
Publication date 2001-05
ISSN 0887-6924
Keyword(s) C/EBP
G-CSF receptor
granulopoiesis
hematopoiesis
cytokines
Summary To assess cooperation between G-CSF signals and C/EBP, we characterized Ba/F3 pro-B cell lines expressing C/EBPWT-ER and the G-CSF receptor (GCSFR). In these lines, GCSFR signals can be evaluated independent of their effect on C/EBP levels. G-CSF alone did not induce the MPO, NE, LF, or PU.1 RNAs, and C/EBPWT-ER alone stimulated low-level MPO and high-level PU.1 expression. Simultaneous activation of the GCSFR and C/EBPWT-ER markedly increased MPO and NE induction at 24 h, and LF mRNA was detected at 48 h. G-CSF did not increase endogenous GCSFR, endogenous C/EBP or exogenous C/EBPWT-ER levels, and C/EBPWT-ER did not induce endogenous or exogenous GCSFR. Several GCSFR mutants were also co-expressed with C/EBPWT-ER. Mutation of all four cytoplasmic tyrosines prevented NE induction but enhanced MPO induction. Mutation of Y704 was required for increased MPO induction. Consistent with this finding, removing IL-3 without G-CSF addition enabled MPO, but not NE, induction by C/EBPWT-ER. GCSFR signals or related signals from other receptors may cooperate with C/EBP to direct differentiation of normal myeloid stem cells.
Language eng
Field of Research 110202 Haematology
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2001, Nature Publishing Group
Persistent URL http://hdl.handle.net/10536/DRO/DU:30009178

Document type: Journal Article
Collection: School of Medicine
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