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Metallothionein 2A expression is associated with cell proliferation in breast cancer

Jin, Rongxian, Chow, Vincent T. K., Tan, Puay-Hoon, Dheen, S. Thameem, Duan, Wei and Bay, Boon-Huat 2002, Metallothionein 2A expression is associated with cell proliferation in breast cancer, Carcinogenesis, vol. 23, no. 1, pp. 81-86.

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Title Metallothionein 2A expression is associated with cell proliferation in breast cancer
Author(s) Jin, Rongxian
Chow, Vincent T. K.
Tan, Puay-Hoon
Dheen, S. Thameem
Duan, Wei
Bay, Boon-Huat
Journal name Carcinogenesis
Volume number 23
Issue number 1
Start page 81
End page 86
Publisher Oxford University Press
Place of publication Oxford, England
Publication date 2002-01
ISSN 0143-3334
Summary Metallothioneins (MTs) belong to a family of cysteine-rich, metal-binding intracellular proteins, which have been linked with cell proliferation. In this study, expression levels of the 8 known MT-1 and MT-2 functional isoforms in human invasive ductal breast cancer specimens were determined by RT–PCR. The expression profiles of the MT protein and MT-2A mRNA were further evaluated in 79 cases of human invasive ductal breast carcinoma by immunohistochemistry and in situ hybridization, and correlated with cancer cell proliferation (determined by Ki-67 nuclear antigen immunolabeling). MT-1A, MT-1E, MT-1F, MT-1G, MT-1H, MT-1X and MT-2A but not MT-1B, were detected in breast cancer tissue samples. The MT-2A mRNA transcript was the highest among all the isoforms detected. A positive correlation was observed between MT-2A mRNA and MT protein expression with Ki-67 labeling (P = 0.0003 and P < 0.0001, respectively) but not with apoptosis (P = 0.1244 and P = 0.8189, respectively). Co-localization of the MT protein and Ki-67 nuclear antigen in breast cancer cells was demonstrated by double immunofluorescence staining. There was also significantly higher MT protein and MT-2A mRNA expression in histological grade 3 tumors than in histological grade 1 and 2 tumors. The finding that MT 2A appears to be the main isoform associated with cell proliferation in invasive ductal breast cancer tissues, may have therapeutic implications.
Language eng
Field of Research 111299 Oncology and Carcinogenesis not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2002, Oxford University Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30009265

Document type: Journal Article
Collection: School of Medicine
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