Retinal sensitivity loss in third-generation n-3 PUFA-deficient rats

Weisinger, Harrison, Armitage, James, Jeffrey, Brett, Mitchell, Drake, Moriguchi, Toru, Sinclair, Andrew, Weisinger, Richard and Salem, Norman 2002, Retinal sensitivity loss in third-generation n-3 PUFA-deficient rats, Lipids, vol. 37, no. 8, pp. 759-765.

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Title Retinal sensitivity loss in third-generation n-3 PUFA-deficient rats
Author(s) Weisinger, Harrison
Armitage, James
Jeffrey, Brett
Mitchell, Drake
Moriguchi, Toru
Sinclair, Andrew
Weisinger, Richard
Salem, Norman
Journal name Lipids
Volume number 37
Issue number 8
Start page 759
End page 765
Publisher American Oil Chemists' Society
Place of publication Champaign, Ill.
Publication date 2002-08
ISSN 0024-4201
1558-9307
Summary A previous study conducted in guinea pigs suggested that ingestion of diets high in EPA and DHA may result in suboptimal retinal function. The aim of the present study was to evaluate retinal function in pigmented (Long-Evans) rats, raised to a third generation on diets that were either deficient in n-3 PUFA or adequate (with the addition of DHA). Electroretinographic assessment employed full-field white flash stimulation. Photoreceptor responses were evaluated in terms of peak amplitudes and implicit times (a-wave, b-wave), intensity-response functions (Naka-Rushton), and the parameters of a model of transduction (P3). Retinal phospholipid FA composition was measured by capillary GLC. DHA levels were reduced by 55% in n-3-deficient animals compared with the n-3-adequate group, whereas the levels of docosapentaenoic acid n-6 were 44 times higher in n-3-deficient animals. The level of arachidonic acid was marginally higher (12.8%) in n-6-adequate animals. The n-3-deficient animals exhibited significantly reduced retinal sensitivity (σ and S values were both affected by 0.29 log units) and increased b-wave implicit times compared with those fed the n-3-adequate diet. These data suggest that n-3 PUFA are required for development of retinal sensitivity, more so than other indices of retinal function assessed by current methods, such as maximal response amplitude. However, the benefit for retinal function of adding preformed DHA to diets already replete in n-3 PUFA remains unclear.
Language eng
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2002, AOCS Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30009296

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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