Biliary intervention augments chemotactic reaction and aggravates cholestatic liver injury in rats

Chuang, Jiin-Haur, Chang, Nyuk-Kong, Huang, Chao-Cheng, Lo, Sing Kai, Wang, Hsiu-Chuan, Chen, Ching-Mei, Wu, Chia-Ling, Chou, Ming-Huei and Lin, Tsu-Kung 2004, Biliary intervention augments chemotactic reaction and aggravates cholestatic liver injury in rats, Journal of surgical research, vol. 120, no. 2, pp. 210-218, doi: 10.1016/j.jss.2004.03.020.

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Title Biliary intervention augments chemotactic reaction and aggravates cholestatic liver injury in rats
Author(s) Chuang, Jiin-Haur
Chang, Nyuk-Kong
Huang, Chao-Cheng
Lo, Sing Kai
Wang, Hsiu-Chuan
Chen, Ching-Mei
Wu, Chia-Ling
Chou, Ming-Huei
Lin, Tsu-Kung
Journal name Journal of surgical research
Volume number 120
Issue number 2
Start page 210
End page 218
Publisher Academic Press
Place of publication New York, N.Y.
Publication date 2004-08
ISSN 0022-4804
Keyword(s) chemokine
macrophage inflammatory protein-2 (MIP-2)
monocyte chemoattractant protein-1 (MCP-1)
obstructive jaundice
Summary Background
Intervention of the biliary system is frequently done in patients with obstructive jaundice and is associated with significant morbidity and mortality. The pathogenesis is unknown.
Materials and methods
A rat model of bile duct ligation (BDL) for 2 weeks was established in which biliary intervention was feasible by injection of normal saline through an indwelling catheter in the bile ducts. Plasma levels of C-C chemokine MCP-1 and C-X-C chemokine MIP-2 were measured by using ELISA. Blood monocytes, Kupffer cells, and neutrophils in the liver were characterized with antibodies to ED1, ED2, and myeloperoxidase (MPO). Lipid peroxidation was measured by malondialdehyde contents and apoptosis by TUNEL stain of the liver.
Biliary intervention resulted in an increase of plasma MCP-1 and MIP-2 proteins by 1 h, which declined to normal level by 3 h in both sham and BDL rats. The levels in BDL rats were significantly higher than in sham at most points. There was a transient increase of ED1- and ED2-positive cells and MPO-staining cells in sham rat liver by 1 h after intervention. ED2-positive cells increased significantly by 1 h, while ED1- and MPO-positive cells decreased, yet insignificantly after intervention in BDL rats. The cell counts in BDL were constantly higher than in sham. Malondialdehyde increased precipitously in BDL by 3 h and was significantly higher than in sham throughout the study period. Parenchymal liver injury, manifested by elevated ALT, as well as apoptosis and necrosis of liver cells, was significantly increased in BDL rats, but not in sham rats.
Biliary intervention augments chemokine expression, precipitates lipid peroxidation, and aggravates liver injury in cholestatic rats.
Language eng
DOI 10.1016/j.jss.2004.03.020
Field of Research 110307 Gastroenterology and Hepatology
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2004, Elsevier
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Document type: Journal Article
Collections: Faculty of Health
School of Psychology
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