High strain mechanical loading rapidly induces tendon apoptosis : an ex vivo rat tibialis anterior model

Scott, A., Khan, K.M., Heer, J., Cook, J. L., Lian, O. and Duronio, V 2005, High strain mechanical loading rapidly induces tendon apoptosis : an ex vivo rat tibialis anterior model, British journal of sports medicines, vol. 39, no. 5, pp. 1-4.

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Title High strain mechanical loading rapidly induces tendon apoptosis : an ex vivo rat tibialis anterior model
Author(s) Scott, A.
Khan, K.M.
Heer, J.
Cook, J. L.
Lian, O.
Duronio, V
Journal name British journal of sports medicines
Volume number 39
Issue number 5
Start page 1
End page 4
Publisher BMJ Publishing Group
Place of publication London, England
Publication date 2005-05
ISSN 0306-3674
Keyword(s) apoptosis
mechanical loading
overuse
rat
tendon
Summary Background: The role of apoptosis, or programmed cell death, has only recently been explored in tendon.

Objective: To investigate the development of apoptosis after high strain loading of rat tendon.

Methods: The right tibialis anterior tendons of three rats were prepared for mechanical loading, and left tendons were prepared identically as non-loaded controls. Tendon was loaded with 20% strain for six hours using a 1 Hz longitudinal sine wave signal. The following were used to assess apoptosis: (a) a monoclonal mouse antibody (F7-26) to label single stranded DNA breaks; (b) a rabbit polyclonal antibody that specifically recognises the cleaved form of caspase-3.

Results: Light microscopy confirmed that the high strain protocol induced a stretch overload injury. Control tendons showed little or no staining with the F7-26 antibody, but the loaded tendons displayed numerous apoptotic cells. The percentage of apoptotic cells (20%) in the loaded tendon was significantly greater than in the control tendon (1%) (p = 0.000). The labelled cells colocalised with abnormal nuclear morphology, including nuclear fragmentation. The staining against cleaved caspase-3 was positive in loaded tendons only, and localised both to nucleus and cytoplasm.

Conclusion:
This experiment extends knowledge of human tendon apoptosis by showing that apoptosis can occur in response to short term, high strain mechanical loading. This is the first report of mechanical loading of intact tendon causing excessive apoptosis.

Language eng
Field of Research 110320 Radiology and Organ Imaging
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2005, BMJ Publishing Group Ltd & British Association of Sport and Exercise Medicine
Persistent URL http://hdl.handle.net/10536/DRO/DU:30009400

Document type: Journal Article
Collections: School of Psychology
School of Exercise and Nutrition Sciences
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