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Elucidation of bucephalid parasite life-cycles using mutation scanning analysis

Hutson, Kate, Styan, Craig, Beveridge, Ian, Keough, Michael, Zhu, Xingquan, Abs El-Osta, Youssef and Gasser, Robin 2004, Elucidation of bucephalid parasite life-cycles using mutation scanning analysis, Molecular and cellular probes, vol. 18, no. 2, pp. 139-146, doi: 10.1016/j.mcp.2003.11.004.

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Title Elucidation of bucephalid parasite life-cycles using mutation scanning analysis
Author(s) Hutson, Kate
Styan, Craig
Beveridge, Ian
Keough, Michael
Zhu, Xingquan
Abs El-Osta, Youssef
Gasser, Robin
Journal name Molecular and cellular probes
Volume number 18
Issue number 2
Start page 139
End page 146
Publisher Academic Press
Place of publication London, England
Publication date 2004-04
ISSN 0890-8508
1096-1194
Keyword(s) Scallop
Digenea
Bucephalid
Identification
Host specificity
Genetic variation
Single-strand conformation polymorphism analysis
Mitochondrial DNA
Summary Nucleotide variation in a portion of the mitochondrial cytochrome c oxidase subunit1 (cox1) gene from asexual stages of bucephalids of southern Australian scallops (Chlamys asperrima, Chlamys bifrons and Pecten fumatus) was investigated using a mutation scanning–sequencing approach. Single-strand conformation polymorphism (SSCP) analysis revealed three main profile types (A, B and C) for parasites isolated from scallops. Sequence analysis revealed that samples represented by profiles B and C had a high degree (97.3%) of sequence similarity, whereas they were ~21% different in sequence from those represented by profile A. These findings suggested that at least two types or species (represented by profile A, or profile B or C) of bucephalid infect scallops, of which both were detected in South Australia, while only one was found in Victoria. The prevalence of bucephalids (and their SSCP haplotypes) appeared to differ among the three species of scallop in South Australia as well as between the two scallop species in Victoria, indicating a degree of host specificity. Adult bucephalids were collected from Eastern Australian Salmon (Arripis trutta), in an attempt to match them with the asexual stages from the scallop hosts. Neither of the two taxa of adult bucephalid (Telorhynchus arripidis and an un-named Telorhynchus species) shared SSCP profiles with the bucephalids from scallops, but were genetically similar, suggesting that the asexual stages from scallops may represent the genus Telorhynchus. This study, which assessed nucleotide sequence variation in a portion of the mitochondrial cox1 gene for bucephalids found in scallops and arripid fish, illustrates the usefulness of the mutation scanning approach to elucidate complex life-cycles of marine parasites.
Language eng
DOI 10.1016/j.mcp.2003.11.004
Field of Research 060411 Population, Ecological and Evolutionary Genetics
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2003, Elsevier Ltd
Persistent URL http://hdl.handle.net/10536/DRO/DU:30009468

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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