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Extracellular matrix induces formation of organoids and changes in cell surface morphology in cultured human breast carcinoma cells PMC42-LA

Ackland, Leigh, Ward, John, Ackland, Christopher, Greaves, Mark and Walker, Mary 2003, Extracellular matrix induces formation of organoids and changes in cell surface morphology in cultured human breast carcinoma cells PMC42-LA, In vitro cellular and development biology - animal, vol. 39, no. 10, pp. 428-433.

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Title Extracellular matrix induces formation of organoids and changes in cell surface morphology in cultured human breast carcinoma cells PMC42-LA
Author(s) Ackland, LeighORCID iD for Ackland, Leigh orcid.org/0000-0002-7474-6556
Ward, John
Ackland, Christopher
Greaves, Mark
Walker, Mary
Journal name In vitro cellular and development biology - animal
Volume number 39
Issue number 10
Start page 428
End page 433
Publisher Society for In Vitro Biology
Place of publication Raleigh, N.C.
Publication date 2003-11
ISSN 1071-2690
Summary In the lactating breast, the development of secretory alveoli consisting of differentiated cells arranged around a central lumen is dependent on signals from the extracellular environment of the cells. There are few cell lines that model this process. We previously showed that the human breast carcinoma line PMC42-LA can be induced to form organoids, reminiscent of secretory alveoli found in the lactating human breast. In this report, we used high-resolution scanning electron microscopy to show that the formation of organoids is accompanied by development of cell surface microvilli. Extracellular matrix-induced formation of microvilli occurred on the internal and external surfaces of cells in the organoids and not on surfaces in contact with the extracellular matrix. Organoid formation of PMC42-LA cells induced a rearrangement of the extracellular matrix, seen in the form of radiating fibers from the organoids. In summary, there is an interaction between PMC42-LA cells and the underlying extracellular matrix, which leads to the formation of polarized cells with well-developed microvilli. This is accompanied by organization of the extracellular matrix. PMC42-LA is a relevant model of the human breast for investigations into cell-cell and cell-matrix interactions.
Language eng
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30012982

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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