Squalene supplementation alters genes associated with liver cholesterol metabolism

Janevski, M., McGlynn, M. and Lewandowski, Paul 2006, Squalene supplementation alters genes associated with liver cholesterol metabolism, Asia Pacific journal of clinical nutrition, vol. 15, no. Supp. 3, pp. S105-S105.

Attached Files
Name Description MIMEType Size Downloads

Title Squalene supplementation alters genes associated with liver cholesterol metabolism
Author(s) Janevski, M.
McGlynn, M.
Lewandowski, Paul
Journal name Asia Pacific journal of clinical nutrition
Volume number 15
Issue number Supp. 3
Start page S105
End page S105
Publisher HEC Press
Place of publication [McKinnon, Vic.]
Publication date 2006
ISSN 0964-7058
Summary Background – Squalene is a component of shark liver oil and has been speculated to have cholesterol reducing properties. High levels of total and LDL cholesterol have been shown to contribute to the development of chronic heart disease. The liver is central to the regulation of cholesterol metabolism and dietary intervention has long been recognized as a primary means to reduce the risks of chronic heart disease and related ailments.
Objectives – To determine the effect of dietary squalene supplementation on gene transcripts associated with liver cholesterol metabolism. Specifically the effect of squalene supplementation on mRNA levels for proteins that
regulate cholesterol biosynthesis (HMDH & ERG1), cholesterol elimination (SRB1), bile synthesis (CP7A1 & CP27A) and cholesterol excretion by the liver into bile (ABCG5 & ABCG8) was investigated.
Design – Rats (n=32) were divided into four groups and supplemented for 12 weeks. Groups one and two were fed a cholesterol rich diet for six weeks followed by six weeks of a cholesterol rich diet plus 1.75mg/day of squalene or 3.5 mg/day. Group three was fed a cholesterol rich diet for 12 weeks and group four was fed standard rat chow for 12 weeks. Blood lipid levels were monitored during the study and liver gene expression was determined at the
conclusion of the feeding trial via RT-PCR.
Outcomes – 3.5 mg/day of squalene lowered total and LDL cholesterol in rats consuming a cholesterol rich diet. This dose of squalene also resulted in constant levels of HMDH and ERG1 whereas the cholesterol rich diet halved mRNA levels of these enzymes. Furthermore 3.5 mg/day of squalene caused a greater than 3.0 fold increase in mRNA levels of the proteins SRB1, CP7A1, CP27A and ABCG5.
Conclusion – Dietary squalene supplementation at a dose of 3.5 mg/day lowers total and LDL cholesterol in rats consuming a cholesterol rich diet. These reductions in cholesterol levels may be due to increased cholesterol
elimination, bile synthesis and cholesterol excretion by the liver into bile mediated by changes in gene expression of key enzymes involved in these metabolic pathways
Notes Reproduced with the specific permission of the copyright owner.
Language eng
Field of Research 111199 Nutrition and Dietetics not elsewhere classified
HERDC Research category C3 Non-refereed articles in a professional journal
Copyright notice ©2006, HEC Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30013451

Document type: Journal Article
Collection: School of Medicine
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Access Statistics: 694 Abstract Views, 51 File Downloads  -  Detailed Statistics
Created: Tue, 21 Oct 2008, 11:27:19 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.