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Interaction of Arabidopsis Thaliana with Plasmodiophora Brassicae

Agarwal, A., Kaul, V., Faggian, R. and Cahill, D. 2006, Interaction of Arabidopsis Thaliana with Plasmodiophora Brassicae, in ComBio 2006 : Proceedings of the Australian Society for Biochemistry and Molecular Biology Combined Conference, Australian Society for Biochemistry and Molecular Biology Inc, Kent Town, S.Aust., pp. 137-137.

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Title Interaction of Arabidopsis Thaliana with Plasmodiophora Brassicae
Author(s) Agarwal, A.
Kaul, V.
Faggian, R.
Cahill, D.
Conference name Australian Society for Biochemistry and Molecular Biology Combined Conference (2006 : Brisbane, Qld.)
Conference location Brisbane, Qld.
Conference dates 24-28 Sep. 2006
Title of proceedings ComBio 2006 : Proceedings of the Australian Society for Biochemistry and Molecular Biology Combined Conference
Publication date 2006
Start page 137
End page 137
Publisher Australian Society for Biochemistry and Molecular Biology Inc
Place of publication Kent Town, S.Aust.
Summary Plasmodiophora brassicae is a protistan pathogen that attacks roots of brassicaceous plant species causing devastating disease. Resistance is characterised by restriction of the pathogen and susceptibility by the development of severely malformed roots (‘clubroots’) and stunting of the plant that is associated with alterations in the synthesis of cytokinin and auxin hormones. We are examining the susceptible response in Arabidopsis and whether suppression of key resistance factors by the pathogen contributes to susceptibility. The interaction is being studied using a number of approaches including microscopy of the infection process and development of the pathogen within roots and host gene expression analysis. Quantitative PCR was used to confirm the timing of infection of roots and showed that infection occurred at day four and colonisation increased thereafter to high levels by 23 days after inoculation by which time roots were showing systemic abnormalities. To investigate the basis of this compatible interaction we have conducted a time course experiment following infection of a susceptible ecotype of Arabidopsis (Col-0) to examine whole genome geneexpression changes in the host. Differential gene expression analysis of inoculated versus control roots showed that a higher number of genes had altered expression levels at day four compared to that at day seven and at day ten. At day four the expression levels of several genes known to be important for recognition and signal transduction in resistant interactions and genes involved in the biosynthesis of lignin, phenylpropanoids and ethylene were suppressed. Suppression by P. brassicae of specific plant defence responses appears to be a key component of susceptibility in this system.
Notes Reproduced with the kind permission of the copyright owner.
Language eng
Field of Research 060704 Plant Pathology
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category E3 Extract of paper
Copyright notice ©2006, Australian Society for Biochemistry and Molecular Biology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30014698

Document type: Conference Paper
Collections: School of Life and Environmental Sciences
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