The presence of GABA in gastropod mucus and its role in inducing larval settlement

Laimek, Praphaporn, Clark, Shona, Stewart, Michael, Pfeffer, Fred, Chaitip, Wanichanona, Hanna, Peter and Sobhon, Prasert 2008, The presence of GABA in gastropod mucus and its role in inducing larval settlement, Journal of experimental marine biology and ecology, vol. 354, no. 2, pp. 182-191, doi: 10.1016/j.jembe.2007.11.003.

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Title The presence of GABA in gastropod mucus and its role in inducing larval settlement
Author(s) Laimek, Praphaporn
Clark, Shona
Stewart, Michael
Pfeffer, FredORCID iD for Pfeffer, Fred
Chaitip, Wanichanona
Hanna, Peter
Sobhon, Prasert
Journal name Journal of experimental marine biology and ecology
Volume number 354
Issue number 2
Start page 182
End page 191
Total pages 10
Publisher North-Holland
Place of publication Amsterdam, The Netherlands
Publication date 2008-01-18
ISSN 0022-0981
Keyword(s) abalone
larval settlement
Summary Chemical substances that induce larval settlement have been the focus of many gastropod studies due to the importance of wild stock recruitment and production within aquaculture facilities. Gamma-aminobutyric acid (GABA), GABA analogs, and GABA-mimetics associated with certain crustose coralline algae (CCA), are known to induce larval settlement in commercial abalone (Haliotis) species, and other gastropods. Furthermore, mucus secreted from these gastropods has been shown to induce larval settlement, but the stimulatory components of mucus have not been thoroughly investigated. We now present data confirming that GABA is the settlement-inducing effector molecule contained within abalone mucus. To do this, we initially generated anti-GABA for use in immunoenzyme and immunofluorescent microscopy. Using these techniques GABA was identified in the nerves and epithelial cells of the foot, including mucus. Dried mucus samples subject to HPLC analysis revealed a mean concentration of 0.68 mM GABA after sample rehydration. The presence of GABA in these samples was confirmed by time-of-flight mass spectroscopy (TOF-MS). In addition, GABA was detected in the mucus of several abalone species and other gastropods by immunocytochemistry. Subsequent bioassays using both dry and fresh mucus strongly promoted induction of larval settlement.
Language eng
DOI 10.1016/j.jembe.2007.11.003
Field of Research 060805 Animal Neurobiology
HERDC Research category C1 Refereed article in a scholarly journal
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