A reservoir of brown adipocyte progenitors in human skeletal muscle

Crisan, Mihaela, Casteilla, Louis, Lehr, Lorenz, Carmona, Mamen, Paolini-Giacobino, Ariane, Yap, Solomon, Sun, Bin, Léger, Bertrand, Logar, Alison, Penicaud, Luc, Schrauwen, Patrick, Cameron-Smith, David, Russell, Aaron Paul, Peault, Bruno and Giacobino, Jean-Paul 2008, A reservoir of brown adipocyte progenitors in human skeletal muscle, Stem cells : the international journal of cell differentiation and proliferation, vol. 26, no. 9, pp. 2425-2433, doi: 10.1634/stemcells.2008-0325.

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Title A reservoir of brown adipocyte progenitors in human skeletal muscle
Author(s) Crisan, Mihaela
Casteilla, Louis
Lehr, Lorenz
Carmona, Mamen
Paolini-Giacobino, Ariane
Yap, Solomon
Sun, Bin
Léger, Bertrand
Logar, Alison
Penicaud, Luc
Schrauwen, Patrick
Cameron-Smith, David
Russell, Aaron PaulORCID iD for Russell, Aaron Paul orcid.org/0000-0002-7323-9501
Peault, Bruno
Giacobino, Jean-Paul
Journal name Stem cells : the international journal of cell differentiation and proliferation
Volume number 26
Issue number 9
Start page 2425
End page 2433
Total pages 9
Publisher AlphaMed Press
Place of publication Dayton, Oh.
Publication date 2008-09
ISSN 1066-5099
Keyword(s) brown adipocytes
human muscle
Summary Brown adipose tissue uncoupling protein-1 (UCP1) plays a major role in the control of energy balance in rodents. It has long been thought, however, that there is no physiologically relevant UCP1 expression in adult humans. In this study we show, using an original approach consisting of sorting cells from various tissues and differentiating them in an adipogenic medium, that a stationary population of skeletal muscle cells expressing the CD34 surface protein can differentiate in vitro into genuine brown adipocytes with a high level of UCP1 expression and uncoupled respiration. These cells can be expanded in culture, and their UCP1 mRNA expression is strongly increased by cell-permeating cAMP derivatives and a peroxisome-proliferator-activated receptor-{gamma} (PPAR{gamma}) agonist. Furthermore, UCP1 mRNA was detected in the skeletal muscle of adult humans, and its expression was increased in vivo by PPAR{gamma} agonist treatment. All the studies concerning UCP1 expression in adult humans have until now been focused on the white adipose tissue. Here we show for the first time the existence in human skeletal muscle and the prospective isolation of progenitor cells with a high potential for UCP1 expression. The discovery of this reservoir generates a new hope of treating obesity by acting on energy dissipation.
Language eng
DOI 10.1634/stemcells.2008-0325
Field of Research 060602 Animal Physiology - Cell
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID NHMRC 479536
HERDC collection year 2008
Copyright notice ©2008, AlphaMed Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30017225

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