ATP7B expression in human breast epithelial cells is mediated by lactational hormones

Michalczyk, Agnes, Bastow, Edward, Greenough, Mark, Camakaris, James, Freestone, David, Taylor, Philip, Linder, Maria, Mercer, Julian and Ackland, M. Leigh 2008, ATP7B expression in human breast epithelial cells is mediated by lactational hormones, Journal of histochemistry and cytochemistry, vol. 56, no. 4, pp. 389-399, doi: 10.1369/jhc.7A7300.2008.

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Title ATP7B expression in human breast epithelial cells is mediated by lactational hormones
Author(s) Michalczyk, AgnesORCID iD for Michalczyk, Agnes
Bastow, Edward
Greenough, Mark
Camakaris, James
Freestone, David
Taylor, PhilipORCID iD for Taylor, Philip
Linder, Maria
Mercer, Julian
Ackland, M. LeighORCID iD for Ackland, M. Leigh
Journal name Journal of histochemistry and cytochemistry
Volume number 56
Issue number 4
Start page 389
End page 399
Total pages 11
Publisher Histochemical Society
Place of publication Seattle, Wash.
Publication date 2008-04
ISSN 0022-1554
Keyword(s) ATP7B
mammary gland
lactational hormones
Summary A role for the copper transporter, ATP7B, in secretion of copper from the human breast into milk has previously not been reported, although it is known that the murine ortholog of ATP7B facilitates copper secretion in the mouse mammary gland. We show here that ATP7B is expressed in luminal epithelial cells in both the resting and lactating human breast, where it has a perinuclear localization in resting epithelial cells and a diffuse location in lactating tissue. ATP7B protein was present in a different subset of vesicles from those containing milk proteins and did not overlap with Menkes ATPase, ATP-7A, except in the perinuclear region of cells. In the cultured human mammary line, PMC42-LA, treatment with lactational hormones induced a redistribution of ATP7B from a perinuclear region to a region adjacent, but not coincident with, the apical plasma membrane. Trafficking of ATP7B was copper dependent, suggesting that the hormone-induced redistribution of ATP7A was mediated through an increase in intracellular copper. Radioactive copper (64Cu) studies using polarized PMC42-LA cells that overexpressed mAtp7B protein showed that this transporter facilitates copper efflux from the apical surface of the cells. In summary, our results are consistent with an important function of ATP7B in the secretion of copper from the human mammary gland.
Notes First publishedin the Journal of Histochemistry and Cytochemistry by the Histochemical Society
Language eng
DOI 10.1369/jhc.7A7300.2008
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2008
Copyright notice ©2008, The Histochemical Society
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