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Copper transport during lactation in transgenic mice expressing the human ATP7A protein

Llanos, Roxana, Michalczyk, Agnes, Freestone, David, Currie, Scott, Linder, Maria C., Ackland, M. Leigh and Mercer, Julian 2008, Copper transport during lactation in transgenic mice expressing the human ATP7A protein, Biochemical and biophysical research communications, vol. 372, no. 4, pp. 613-617, doi: 10.1016/j.bbrc.2008.05.123.

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Title Copper transport during lactation in transgenic mice expressing the human ATP7A protein
Author(s) Llanos, Roxana
Michalczyk, AgnesORCID iD for Michalczyk, Agnes orcid.org/0000-0001-5716-0783
Freestone, David
Currie, Scott
Linder, Maria C.
Ackland, M. LeighORCID iD for Ackland, M. Leigh orcid.org/0000-0002-7474-6556
Mercer, Julian
Journal name Biochemical and biophysical research communications
Volume number 372
Issue number 4
Start page 613
End page 617
Total pages 5
Publisher Academic Press
Place of publication San Diego, Calif.
Publication date 2008-08-08
ISSN 0006-291X
1090-2104
Keyword(s) ATP7A
ATP7B
copper
lactation
mammary gland
Summary Both copper transporting ATPases, ATP7A and ATP7B, are expressed in mammary epithelial cells but their role in copper delivery to milk has not been clarified. We investigated the role of ATP7A in delivery of copper to milk using transgenic mice that over-express human ATP7A. In mammary gland of transgenic mice, human ATP7A protein was 10- to 20-fold higher than in control mice, and was localized to the basolateral membrane of mammary epithelial cells in lactating mice. The copper concentration in the mammary gland of transgenic dams and stomach contents of transgenic pups was significantly reduced compared to non-transgenic mice. The mRNA levels of endogenous Atp7a, Atp7b, and Ctr1 copper transporters in the mammary gland were not altered by the expression of the ATP7A transgene, and the protein levels of Atp7b and ceruloplasmin were similar in transgenic and non-transgenic mice. These data suggest that ATP7A plays a role in removing excess copper from the mammary epithelial cells rather than supplying copper to milk.
Language eng
DOI 10.1016/j.bbrc.2008.05.123
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2008
Copyright notice ©2008, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30017279

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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