Characterization of Campylobacter concisus hemolysins

Istivan, Taghrid S., Smith, Stuart, Fry, Benjamin N. and Coloe, Peter J. 2008, Characterization of Campylobacter concisus hemolysins, FEMS immunology and medical microbiology, vol. 54, no. 2, pp. 224-235.

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Title Characterization of Campylobacter concisus hemolysins
Formatted title Characterization of Campylobacter concisus hemolysins
Author(s) Istivan, Taghrid S.
Smith, Stuart
Fry, Benjamin N.
Coloe, Peter J.
Journal name FEMS immunology and medical microbiology
Volume number 54
Issue number 2
Start page 224
End page 235
Total pages 12
Publisher Wiley-Blackwell
Place of publication Oxford, England
Publication date 2008-11
ISSN 0928-8244
1574-695X
Keyword(s) Campylobacter concisus
secreted hemolysins
cytotoxins
membrane bound
OMPLA
pldA gene
Summary Campylobacter concisus is an opportunistic pathogen commonly found in the human oral cavity. It has also been isolated from clinical sources including gastroenteritis cases. Both secreted and cell-associated hemolytic activities were detected in C. concisus strains isolated from children with gastroenteritis. The secreted hemolytic activity of C. concisus strains was labile and was detected in variable levels from fresh-culture filtrates only. In addition, another secreted hemolysin/cytotoxin with a molecular weight <10 kDa was detected in a single C. concisus strain (RCH 12). A C. concisus genomic library, constructed from strain RCH 3 in Escherichia coli XL1-Blue, was screened for hemolytic clones. Subcloning and sequence analysis of selected hemolytic clones identified ORFs for genes that enhance hemolytic activity but do not appear to be related to any known hemolysin genes found in Gram-negative bacteria. In a previous study, a stable cell-associated hemolysin was identified as an outer-membrane phospholipase A (OMPLA) encoded by the pldA gene. In this study, we report cloning of the pldA gene of the clinical strain C. concisus RCH 3 and the complementation of phospholipase A activity in an E. coli pldA mutant.
Language eng
Field of Research 119999 Medical and Health Sciences not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2008
Copyright notice ©2008, Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
Persistent URL http://hdl.handle.net/10536/DRO/DU:30017282

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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