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Conjugated linoleic acid suppresses myogenic gene expression in a model of human muscle cell inflammation

Larsen, Amy E., Cameron-Smith, David and Crowe, Timothy 2008, Conjugated linoleic acid suppresses myogenic gene expression in a model of human muscle cell inflammation, Journal of nutrition, vol. 138, no. 1, pp. 12-16.

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Title Conjugated linoleic acid suppresses myogenic gene expression in a model of human muscle cell inflammation
Author(s) Larsen, Amy E.
Cameron-Smith, David
Crowe, Timothy
Journal name Journal of nutrition
Volume number 138
Issue number 1
Start page 12
End page 16
Publisher American Society for Nutrition
Place of publication Bethesda, Md.
Publication date 2008-01
ISSN 0022-3166
1541-6100
Summary Proinflammatory cytokines, such as tumor necrosis factor (TNF)-{alpha}, contribute to muscle wasting in inflammatory disorders, where TNF{alpha} acts to regulate myogenic genes. Conjugated linoleic acid (CLA) has shown promise as an antiproliferative and antiinflammatory agent, leading to its potential as a therapeutic agent in muscle-wasting disorders. To evaluate the effect of CLA on myogenesis during inflammation, human primary muscle cells were grown in culture and exposed to varying concentrations of TNF{alpha} and the cis-9, trans-11 and trans-10, cis-12 CLA isomers. Expression of myogenic genes (Myf5, MyoD, myogenin, and myostatin) and the functional genes creatine kinase (CK) and myosin heavy chain (MHC IIx) were measured by real-time PCR. TNF{alpha} significantly downregulated MyoD and myogenin expression, whereas it increased Myf5 expression. These changes corresponded with a decrease in both CK and MHC IIx expression. Both isomers of CLA mimicked the inhibitory effect of TNF{alpha} treatment on MyoD and myogenin expression, whereas myostatin expression was diminished in the presence of both isomers of CLA either alone or in combination with TNF{alpha}. Both isomers of CLA decreased CK and MHC IIx expression. These findings demonstrate that TNF{alpha} can have specific regulatory effects on myogenic genes in primary human muscle cells. A postulated antiinflammatory role of CLA in myogenesis appears more complex, with an indication that CLA may have a negative effect on this process.
Language eng
Field of Research 111299 Oncology and Carcinogenesis not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2008
Copyright notice ©2008, American Society for Nutrition
Persistent URL http://hdl.handle.net/10536/DRO/DU:30017379

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