Global and targeted gene expression and protein content in skeletal muscle of young men following short-term creatine monohydrate supplementation

Safdar, Adeel, Yardley, Nicholas J., Snow, Rodney, Melov, Simon and Tarnopolsky, Mark A. 2008, Global and targeted gene expression and protein content in skeletal muscle of young men following short-term creatine monohydrate supplementation, Physiological genomics, vol. 32, pp. 219-228.

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Title Global and targeted gene expression and protein content in skeletal muscle of young men following short-term creatine monohydrate supplementation
Author(s) Safdar, Adeel
Yardley, Nicholas J.
Snow, Rodney
Melov, Simon
Tarnopolsky, Mark A.
Journal name Physiological genomics
Volume number 32
Start page 219
End page 228
Total pages 10
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2008-01
ISSN 1094-8341
1531-2267
Keyword(s) ergogenic aid
osmosensing
cell signaling
cDNA microarray
real-time PCR
Summary Creatine monohydrate (CrM) supplementation has been shown to increase fat-free mass and muscle power output possibly via cell swelling. Little is known about the cellular response to CrM. We investigated the effect of short-term CrM supplementation on global and targeted mRNA expression and protein content in human skeletal muscle. In a randomized, placebo-controlled, crossover, double-blind design, 12 young, healthy, nonobese men were supplemented with either a placebo (PL) or CrM (loading phase, 20 g/day x 3 days; maintenance phase, 5 g/day x 7 days) for 10 days. Following a 28-day washout period, subjects were put on the alternate supplementation for 10 days. Muscle biopsies of the vastus lateralis were obtained and were assessed for mRNA expression (cDNA microarrays + real-time PCR) and protein content (Kinetworks KPKS 1.0 Protein Kinase screen). CrM supplementation significantly increased fat-free mass, total body water, and body weight of the participants (P < 0.05). Also, CrM supplementation significantly upregulated (1.3- to 5.0-fold) the mRNA content of genes and protein content of kinases involved in osmosensing and signal transduction, cytoskeleton remodeling, protein and glycogen synthesis regulation, satellite cell proliferation and differentiation, DNA replication and repair, RNA transcription control, and cell survival. We are the first to report this large-scale gene expression in the skeletal muscle with short-term CrM supplementation, a response that suggests changes in cellular osmolarity.
Language eng
Field of Research 111601 Cell Physiology
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2008
Copyright notice ©2008, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30017389

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