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Foot mucus is a good source for non-destructive genetic sampling in polyplacophora

Palmer, Amy N. S., Styan, Craig A. and Shearman, Deborah C. A. 2008, Foot mucus is a good source for non-destructive genetic sampling in polyplacophora, Conservation genetics, vol. 9, no. 1, pp. 229-231, doi: 10.1007/s10592-007-9320-4.

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Title Foot mucus is a good source for non-destructive genetic sampling in polyplacophora
Author(s) Palmer, Amy N. S.
Styan, Craig A.
Shearman, Deborah C. A.
Journal name Conservation genetics
Volume number 9
Issue number 1
Start page 229
End page 231
Publisher Springer Netherlands
Place of publication Delft, The Netherlands
Publication date 2008-02
ISSN 1566-0621
1572-9737
Keyword(s) non-destructive sampling
molluscs
mtDNA
ischnochiton
Summary A non-destructive method for collecting samples for DNA analysis from the mucus of molluscs was successfully adapted for use with the genus Ischnochiton. DNA was extracted using a Chelex-based method and the COI subunit of the mtDNA was amplified and sequenced. Sequences from the mucus were crosschecked against sequences from the foot tissue of the same animal and were found to be identical. This method provides a non-destructive way of carrying out larger studies of the genetics of rare organisms and may be of general use for genetic-based field studies of molluscs.
Notes Published online: 20 April 2007
Language eng
DOI 10.1007/s10592-007-9320-4
Field of Research 069999 Biological Sciences not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2007, Springer Science+Business Media B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30017483

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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