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Truncation of Plasmodium berghei merozoite surface protein 8 does not affect in vivo blood-stage development

de Koning-Ward, Tania, Drew, Damien R., Chesson, Joanne M., Beeson, James G. and Crabb, Brendan S. 2008, Truncation of Plasmodium berghei merozoite surface protein 8 does not affect in vivo blood-stage development, Molecular and biochemical parasitology, vol. 159, no. 1, pp. 69-72.

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Title Truncation of Plasmodium berghei merozoite surface protein 8 does not affect in vivo blood-stage development
Formatted title Truncation of Plasmodium berghei merozoite surface protein 8 does not affect in vivo blood-stage development
Author(s) de Koning-Ward, Tania
Drew, Damien R.
Chesson, Joanne M.
Beeson, James G.
Crabb, Brendan S.
Journal name Molecular and biochemical parasitology
Volume number 159
Issue number 1
Start page 69
End page 72
Total pages 4
Publisher Elsevier B.V.
Place of publication Amsterdam, Netherlands
Publication date 2008-05
ISSN 0166-6851
1872-9428
Keyword(s) malaria
Plasmodium berghei
merozoite surface proteins
MSP8
gene knockout
Summary Merozoite surface protein 8 (MSP8) has shown promise as a vaccine candidate in the Plasmodium yoelii rodent malaria model and has a proposed role in merozoite invasion of erythrocytes. However, the temporal expression and localisation of MSP8 are unusual for a merozoite antigen. Moreover, in Plasmodium falciparum the MSP8 gene could be disrupted with no apparent effect on in vitro growth. To address the in vivo function of full-length MSP8, we truncated MSP8 in the rodent parasite Plasmodium berghei. PbΔMSP8 disruptant parasites displayed a normal blood-stage growth rate but no increase in reticulocyte preference, a phenomenon observed in P. yoelii MSP8 vaccinated mice. Expression levels of erythrocyte surface antigens were similar in P. berghei wild-type and PbΔMSP8-infected erythrocytes, suggesting that a parasitophorous vacuole function for MSP8 does not involve global trafficking of such antigens. These data demonstrate that a full-length membrane-associated form of PbMSP8 is not essential for blood-stage growth.
Language eng
Field of Research 110803 Medical Parasitology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
HERDC collection year 2008
Copyright notice ©2008, Elsevier B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30017589

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.