Bovine milk fat enriched in conjugated linoleic and vaccenic acids attenuates allergic airway disease in mice

Kanwar, R., MacGibbon, A., Black, P., Kanwar, J., Rowan, A., Vale, M. and Krissansen, G. 2008, Bovine milk fat enriched in conjugated linoleic and vaccenic acids attenuates allergic airway disease in mice, Clinical and experimental allergy, vol. 38, no. 1, pp. 208-218.

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Title Bovine milk fat enriched in conjugated linoleic and vaccenic acids attenuates allergic airway disease in mice
Author(s) Kanwar, R.
MacGibbon, A.
Black, P.
Kanwar, J.
Rowan, A.
Vale, M.
Krissansen, G.
Journal name Clinical and experimental allergy
Volume number 38
Issue number 1
Start page 208
End page 218
Publisher Wiley-Blackwell Publishing Ltd
Place of publication Oxford, England
Publication date 2008-01
ISSN 0954-7894
1365-2222
Keyword(s) airway disease
airway remodelling
allergen-specific Ig
allergy
CCL11
conjugated linoleic acid (CLA)
eosinophils
IL-5
milk fat
vaccenic acid
Summary Background: It has been argued that a reduction in the Western diet of anti-inflammatory unsaturated lipids, such as n-3 polyunsaturated fatty acids, has contributed to the increase in the frequency and severity of allergic diseases.

Objective
: We investigated whether feeding milk fat enriched in conjugated linoleic acid and vaccenic acids (VAs) ('enriched' milk fat), produced by supplementing the diet of pasture-fed cows with fish and sunflower oil, will prevent development of allergic airway responses.

Methods: C57BL/6 mice were fed a control diet containing soybean oil and diets supplemented with milk lipids. They were sensitized by intraperitoneal injection of ovalbumin (OVA) on days 14 and 28, and challenged intranasally with OVA on day 42. Bronchoalveolar lavage fluid, lung tissues and serum samples were collected 6 days after the intranasal challenge.

Results
: Feeding of enriched milk fat led to marked suppression of airway inflammation as evidenced by reductions in eosinophilia and lymphocytosis in the airways, compared with feeding of normal milk fat and control diet. Enriched milk fat significantly reduced circulating allergen-specific IgE and IgG1 levels, together with reductions in bronchoalveolar lavage fluid of IL-5 and CCL11. Treatment significantly inhibited changes in the airway including airway epithelial cell hypertrophy, goblet cell metaplasia and mucus hypersecretion. The two major components of enriched milk fat, cis-9, trans-11 conjugated linoleic acid and VA, inhibited airway inflammation when fed together to mice, whereas alone they were not effective.

Conclusion
: Milk fat enriched in conjugated linoleic and VAs suppresses inflammation and changes to the airways in an animal model of allergic airway disease.
Language eng
Field of Research 110701 Allergy
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2008
Copyright notice ©2007, The Authors
Persistent URL http://hdl.handle.net/10536/DRO/DU:30018000

Document type: Journal Article
Collection: Institute of Biotechnology
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