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Morphological and biochemical alterations of abalone testicular germ cells and spawned sperm and their fertilizing ability

Suphamungmee, Worawit, Weerachatyanukul, Wattana, Poomtong, Tanes, Hanna, Peter and Sobhon, Prasert 2008, Morphological and biochemical alterations of abalone testicular germ cells and spawned sperm and their fertilizing ability, Marine biotechnology, vol. 10, no. 5, pp. 593-601, doi: 10.1007/s10126-008-9097-6.

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Title Morphological and biochemical alterations of abalone testicular germ cells and spawned sperm and their fertilizing ability
Author(s) Suphamungmee, Worawit
Weerachatyanukul, Wattana
Poomtong, Tanes
Hanna, Peter
Sobhon, Prasert
Journal name Marine biotechnology
Volume number 10
Issue number 5
Start page 593
End page 601
Publisher Springer - Verlag
Place of publication N.Y., N.Y.
Publication date 2008-09
ISSN 1436-2228
1436-2236
Keyword(s) abalone
sperm
capacitation
phosphorylation
acrosomal enzymes
Summary In this study, we aimed to detect morphological and biochemical changes in developing germ cells (Gc), testicular sperm (Tsp), and spawned sperm (Ssp) using capacitation-associated characteristics. Gradual changes in the profiles of two membrane proteins, namely NaCl- and detergent-extractable proteins, were observed as compared Gc with Tsp and Tsp with Ssp. These membrane modifications were accomplished mostly through the introduction of new protein sets, both peripheral and integral, into Tsp and Ssp membranes. Activation of serine proteases, particularly in Ssp detergent-extracted proteins with the molecular masses of 38–130 kDa was evident and marked a major difference between Ssp and Tsp. An increase in the level of tyrosine phosphorylation of the proteins ranging from 15 to 20 kDa was noted in Tsp and remained constant in Ssp. Specifically, these three capacitation-associated characteristics could be detected in Ssp, possessing full fertilizing capacity. The lack of an activated proteolytic activity in Tsp resulted in a delayed fertilization, but not affected fertilizing ability. We believe that these characteristics should be advantageous in predicting abalone sperm fertilizing capability, particularly in cases when isolated germ cells or purified Tsp are used in place of spawned sperm in abalone aquaculture.
Language eng
DOI 10.1007/s10126-008-9097-6
Field of Research 060805 Animal Neurobiology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2008
Copyright notice ©2008, Springer Science + Business Media LLC
Persistent URL http://hdl.handle.net/10536/DRO/DU:30018022

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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