Benchmarking ventricular arrhythmias in the mouse—revisiting the ‘Lambeth Conventions’ 20 years on

Huggins, Catherine E., Bell, James R., Pepe, Salvatore and Delbridge, Lea M.D 2008, Benchmarking ventricular arrhythmias in the mouse—revisiting the ‘Lambeth Conventions’ 20 years on, Heart, lung and circulation, vol. 17, no. 6, pp. 445-450.

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Title Benchmarking ventricular arrhythmias in the mouse—revisiting the ‘Lambeth Conventions’ 20 years on
Author(s) Huggins, Catherine E.
Bell, James R.
Pepe, Salvatore
Delbridge, Lea M.D
Journal name Heart, lung and circulation
Volume number 17
Issue number 6
Start page 445
End page 450
Publisher Blackwell Science Asia
Place of publication Carlton South, Vic.
Publication date 2008-12
ISSN 1443-9506
Keyword(s) arrhythmia
murine ex vivo heart
Langendorff perfusion
ectopy
ischaemia–reperfusion
Summary The isolated Langendorff-mode perfused heart has become a valuable experimental model, used extensively to examine cardiac function, pathophysiology and pharmacology. For the clinical cardiologist an ECG is often a simple practicality, however in experimental circumstances, particularly with ex vivo murine hearts it is not always possible to obtain an ECG due to experimental recording constraints. However, the mechanical record of ventricular contractile function can be highly informative in relation to electrical state. It is difficult though to achieve consistency in these evaluations of arrhythmia as a validated common reference framework is lacking. In 1988, a group of investigators developed the ‘Lambeth Conventions’—a standardised reference for the definition and classification of arrhythmias in animal experimental models of ischaemia, infarction and reperfusion in vivo. Now, two decades later it is timely to revisit the Lambeth Conventions, and to update the guidelines in the context of the marked increase in murine heart study in experimental cardiac pathophysiology. Here we describe an adjunct to the Lambeth Conventions for the reporting of ventricular arrhythmias post-ischaemia in ex vivo mouse hearts when ECG recordings are not employed. Of seven discrete and identifiable patterns of mechanical dysrhythmia observed in reperfusion, five could be classified using conventional ECG terminology: ventricular premature beat, bigeminy, trigeminy, ventricular tachycardia and ventricular fibrillation. Two additional rhythm variations detected from the pressure record are described (potentiated contraction and alternans).
Language eng
Field of Research 110201 Cardiology (incl Cardiovascular Diseases)
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2008, Australasian Society of Cardiac and Thoracic Surgeons and the Cardiac Society of Australia and New Zealand. Published by Elsevier Inc.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30018602

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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