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Isolation and restraint stress results in differential activation of corticontrophin-releasing hormone and arginine vasopressin neurones in sheep

Rivalland, E. T. A., Clarke, I. J., Turner, A. I., Pompolo, S. and Tilbrook, A. J. 2007, Isolation and restraint stress results in differential activation of corticontrophin-releasing hormone and arginine vasopressin neurones in sheep, Neuroscience, vol. 145, no. 3, pp. 1048-1058, doi: 10.1016/j.neuroscience.2006.12.045.

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Title Isolation and restraint stress results in differential activation of corticontrophin-releasing hormone and arginine vasopressin neurones in sheep
Author(s) Rivalland, E. T. A.
Clarke, I. J.
Turner, A. I.ORCID iD for Turner, A. I. orcid.org/0000-0002-0682-2860
Pompolo, S.
Tilbrook, A. J.
Journal name Neuroscience
Volume number 145
Issue number 3
Start page 1048
End page 1058
Publisher Pergamon
Place of publication Oxford, England
Publication date 2007-03-30
ISSN 0306-4522
1873-7544
Keyword(s) enkephalin
paraventricular nucleus
hypothalamo–pituitary–adrenal axis
sex differences
Summary This study investigated sex differences in the stress-induced activation of neurons containing corticotrophin-releasing hormone (CRH), arginine vasopressin (AVP) and enkephalin in the paraventricular nucleus (PVN) of gonadectomized male and female sheep. Groups (n=3) of both sexes were either subjected to 90 min isolation and restraint stress (stress group) or were not stressed. Blood samples were taken every 10 min for 90 min prior to and after stress to monitor cortisol levels in plasma. Brains were harvested after 90 min of stress. Stress caused elevation of plasma cortisol levels to a similar extent in both sexes. Double-labeling immunohistochemistry for Fos and either CRH, AVP or enkephalin was undertaken to quantify the numbers of neurons staining for CRH, AVP and enkephalin that also immunostained for Fos. Stress increased Fos immunostaining in all cell types. There was a greater proportion of CRH than AVP neurons activated in stressed animals. There were no sex differences in the activation of CRH and AVP neurons although females had a greater proportion of enkephalin cells staining for Fos than males in both control and stressed animals. There were no differences between control and stressed animals in the proportion of cells co-staining for CRH and AVP. We conclude that isolation and restraint stress activates neurons producing CRH, AVP and enkephalin in sheep and that CRH may play a greater role than AVP in regulating adrenocorticotrophic hormone secretion in response to this stressor in sheep. Finally, isolation and restraint stress does not influence co-localization of CRH and AVP in sheep.
Language eng
DOI 10.1016/j.neuroscience.2006.12.045
Field of Research 110306 Endocrinology
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006, IBRO Published by Elsevier Ltd
Persistent URL http://hdl.handle.net/10536/DRO/DU:30018604

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Created: Wed, 09 Sep 2009, 14:43:52 EST by Rachael Wilson

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