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Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species : screening of strains and optimization of omega-3 production

Burja, Adam M., Radianingtyas, Helia, Windust, Anthony and Barrow, Colin J. 2006, Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species : screening of strains and optimization of omega-3 production, Applied microbiology and biotechnology, vol. 72, no. 6, pp. 1161-1169.

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Title Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species : screening of strains and optimization of omega-3 production
Formatted title Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species : screening of strains and optimization of omega-3 production
Author(s) Burja, Adam M.
Radianingtyas, Helia
Windust, Anthony
Barrow, Colin J.
Journal name Applied microbiology and biotechnology
Volume number 72
Issue number 6
Start page 1161
End page 1169
Publisher Springer
Place of publication Heidelberg, Germany
Publication date 2006-10
ISSN 0175-7598
1432-0614
Summary An isolation program targeting Thraustochytrids (marine fungoid protists) from 19 different Atlantic Canadian locations was performed. Sixty-eight isolates were screened for biomass, total fatty acid (TFA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) content. Analysis of fatty acid methyl ester results discerned four distinctive clusters based on fatty acid profiles, with biomass ranging from 0.1 to 2.3 g L−1, and lipid, EPA, and DHA contents ranging from 27.1 to 321.14, 2.97 to 21.25, and 5.18 to 83.63 mg g−1 biomass, respectively. ONC-T18, was subsequently chosen for further manipulations. Identified using 18S rRNA gene sequencing techniques as a Thraustochytrium sp., most closely related to Thraustochytrium striatum T91-6, ONC-T18 produced up to 28.0 g L−1 biomass, 81.7% TFA, 31.4% (w/w biomass) DHA, and 4.6 g L−1 DHA under optimal fermentation conditions. Furthermore, this strain was found to produce the carotenoids and xanthophylls astaxanthin, zeaxanthin, canthaxanthin, echinenone, and β-carotene. Given this strain’s impressive productivity when compared to commercial strains, such as Schizochytrium sp. SR21 (which has only 50% TFA), coupled with its ability to grow at economical nitrogen and very low salt concentrations (2 g L−1), ONC-T18 is seen as an ideal candidate for both scale-up and commercialization.
Language eng
Field of Research 110899 Medical Microbiology not elsewhere classified
100499 Medical Biotechnology not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006, Springer-Verlag
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019478

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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Created: Wed, 16 Sep 2009, 09:12:09 EST by Rachael Mackenzie