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Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species : screening of strains and optimization of omega-3 production Burja, Adam M., Radianingtyas, Helia, Windust, Anthony and Barrow, Colin J. 2006, Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species : screening of strains and optimization of omega-3 production, Applied microbiology and biotechnology, vol. 72, no. 6, pp. 1161-1169. Attached Files Name Description MIMEType Size Downloads Title Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species : screening of strains and optimization of omega-3 production Formatted title Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species : screening of strains and optimization of omega-3 production Author(s) Burja, Adam M. Radianingtyas, Helia Windust, Anthony Barrow, Colin J. Journal name Applied microbiology and biotechnology Volume number 72 Issue number 6 Start page 1161 End page 1169 Publisher Springer Place of publication Heidelberg, Germany Publication date 2006-10 ISSN 0175-7598 1432-0614 Summary An isolation program targeting Thraustochytrids (marine fungoid protists) from 19 different Atlantic Canadian locations was performed. Sixty-eight isolates were screened for biomass, total fatty acid (TFA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) content. Analysis of fatty acid methyl ester results discerned four distinctive clusters based on fatty acid profiles, with biomass ranging from 0.1 to 2.3 g L−1, and lipid, EPA, and DHA contents ranging from 27.1 to 321.14, 2.97 to 21.25, and 5.18 to 83.63 mg g−1 biomass, respectively. ONC-T18, was subsequently chosen for further manipulations. Identified using 18S rRNA gene sequencing techniques as a Thraustochytrium sp., most closely related to Thraustochytrium striatum T91-6, ONC-T18 produced up to 28.0 g L−1 biomass, 81.7% TFA, 31.4% (w/w biomass) DHA, and 4.6 g L−1 DHA under optimal fermentation conditions. Furthermore, this strain was found to produce the carotenoids and xanthophylls astaxanthin, zeaxanthin, canthaxanthin, echinenone, and β-carotene. Given this strain’s impressive productivity when compared to commercial strains, such as Schizochytrium sp. SR21 (which has only 50% TFA), coupled with its ability to grow at economical nitrogen and very low salt concentrations (2 g L−1), ONC-T18 is seen as an ideal candidate for both scale-up and commercialization. Language eng Field of Research 110899 Medical Microbiology not elsewhere classified 100499 Medical Biotechnology not elsewhere classified Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©2006, Springer-Verlag Persistent URL http://hdl.handle.net/10536/DRO/DU:30019478 Document type: Journal Article Collection: School of Life and Environmental Sciences Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Wed, 16 Sep 2009, 09:12:09 EST Wed, 16 Sep 2009, 09:21:13 EST Tue, 29 Sep 2009, 15:33:42 EST Tue, 29 Sep 2009, 15:34:37 EST Tue, 01 Dec 2009, 11:01:49 EST Tue, 01 Dec 2009, 11:11:13 EST Wed, 13 Jan 2010, 12:46:16 EST Thu, 14 Jan 2010, 10:45:47 EST Tue, 08 Oct 2013, 09:06:55 EST Filtered Full Citation counts:   Cited 54 times in TR Web of Science Cited 61 times in Scopus Search Google Scholar Access Statistics: 434 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Wed, 16 Sep 2009, 09:12:09 EST by Rachael Mackenzie

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