Immunostimulatory polysaccharides from Chlorella pyrenoidosa. A new galactofuranan. Measurement of molecular weight and molecular weight dispersion by DOSY NMR.

Suarez, Erick Reyes, Syvitski, Raymond, Kralovec, Jaroslav A., Noseda, Miguel D., Barrow, Colin J., Ewart, H. Stephen, Lumsden, Michael D. and Grindley, T. Bruce 2006, Immunostimulatory polysaccharides from Chlorella pyrenoidosa. A new galactofuranan. Measurement of molecular weight and molecular weight dispersion by DOSY NMR., Biomacromolecules, vol. 7, no. 8, pp. 2368-2376.

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Title Immunostimulatory polysaccharides from Chlorella pyrenoidosa. A new galactofuranan. Measurement of molecular weight and molecular weight dispersion by DOSY NMR.
Formatted title Immunostimulatory polysaccharides from Chlorella pyrenoidosa. A new galactofuranan. Measurement of molecular weight and molecular weight dispersion by DOSY NMR.
Author(s) Suarez, Erick Reyes
Syvitski, Raymond
Kralovec, Jaroslav A.
Noseda, Miguel D.
Barrow, Colin J.
Ewart, H. Stephen
Lumsden, Michael D.
Grindley, T. Bruce
Journal name Biomacromolecules
Volume number 7
Issue number 8
Start page 2368
End page 2376
Publisher American Chemical Society
Place of publication Washington, D.C.
Publication date 2006-08
ISSN 1525-7797
1526-4602
Summary Fractionation of the hot water extract of Chlorella pyrenoidosa was performed using a combination of ethanol precipitation, size exclusion chromatography, and anion exchange chromatography. One fraction contained a new polysaccharide, and this compound was shown to be a 1→2-linked β-d-galactofuranan from its 1D and 2D 1H and 13C NMR spectra, with a molecular weight of 15 kDa from DOSY NMR measurements. A number of other fractions were shown to have the same repeating unit as the previously identified arabinogalactan. However, arabinogalactans from different fractions were shown by DOSY NMR to have different molecular weights, which ranged from 27 to 1020 kDa. Agreement with molecular weights measured for some of these fractions by SEC-MALS was very good, further confirming the relationship established by Viel et al. between molecular weights of neutral polysaccharides and self-diffusion coefficients. The smaller molecular weight polysaccharides, the galactofuranan and the 27 and 50 kDa arabinogalactans, were shown to be close to monodisperse by analysis of the distributions of the self-diffusion coefficients for the polymers. The larger arabinogalactans had considerable variation in their molecular weights (188 ± 109 kDa and 1020 ± 370 kDa). Only the two larger arabinogalactans showed immunostimulatory activity.
Language eng
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006, American Chemical Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019486

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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