Deakin home > Deakin University Library > Deakin Research Online > A simple 96-well microplate method for estimation of total polyphenolic content in seaweeds

A simple 96-well microplate method for estimation of total polyphenolic content in seaweeds

Zhang, Qing, Zhang, Junzeng, Shen, Jingkai, Silva, Angelica, Dennis, Dorothy A. and Barrow, Colin J. 2006, A simple 96-well microplate method for estimation of total polyphenolic content in seaweeds, Journal of applied phycology, vol. 18, no. 3-5, pp. 445-450.

Attached Files (Some files may be inaccessible until you login with your Deakin Research Online credentials)
Name Description MIMEType Size Downloads

Title A simple 96-well microplate method for estimation of total polyphenolic content in seaweeds
Author(s) Zhang, Qing
Zhang, Junzeng
Shen, Jingkai
Silva, Angelica
Dennis, Dorothy A.
Barrow, Colin J.
Journal name Journal of applied phycology
Volume number 18
Issue number 3-5
Start page 445
End page 450
Publisher Kluwer Academic Publishers
Place of publication Dordrecht, Netherlands
Publication date 2006-10
ISSN 0921-8971
1573-5176
0018-8158
Keyword(s) seaweed
marine algae
total polyphenol
estimation
96-well microplate
Summary Seaweed polyphenols are potent antioxidants and have also been shown to have α-glucosidase inhibiting activity. In our continuous efforts to develop new marine-based nutraceuticals and functional food ingredients, we have investigated many algal species collected on the Atlantic coast of Canada. A simple method for estimating the total polyphenol content in seaweeds and their extracts was developed based on the classic Folin-Ciocalteau colorimetric reaction. By using the 96-well microplate and a microplate reader, this new method saves experimental time, significantly reduces the amount of sample required, handles large number of samples in one experiment, and also improves the repeatability of the results.

A number of algal samples collected on the seashore of Nova Scotia, Canada, were analyzed for their levels of polyphenol content using this microplate-based method. The antioxidant activity of these samples was also assessed by using DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging assay. The results showed that there is a strong correlation between the total polyphenol content and the potency of antioxidant effect.
Language eng
Field of Research 090802 Food Engineering
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019489

Document type: Journal Article
Collection: School of Life and Environmental Sciences
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in Deakin Research Online is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 31 times in TR Web of Science
Scopus Citation Count Cited 38 times in Scopus
Access Statistics: 550 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Wed, 16 Sep 2009, 10:04:49 EST by Rachael Mackenzie