Critical assessment of various techniques for the extraction of carotenoids and co-enzyme Q10 from the Thraustochytrid strain ONC-T18

Armenta, Roberto E., Burja, Adam, Radianingtyas, Helia and Barrow, Colin J. 2006, Critical assessment of various techniques for the extraction of carotenoids and co-enzyme Q10 from the Thraustochytrid strain ONC-T18, Journal of agricultural and food chemistry, vol. 54, no. 26, pp. 9752-9758.

Attached Files
Name Description MIMEType Size Downloads

Title Critical assessment of various techniques for the extraction of carotenoids and co-enzyme Q10 from the Thraustochytrid strain ONC-T18
Formatted title Critical assessment of various techniques for the extraction of carotenoids and co-enzyme Q10 from the Thraustochytrid strain ONC-T18
Author(s) Armenta, Roberto E.
Burja, Adam
Radianingtyas, Helia
Barrow, Colin J.
Journal name Journal of agricultural and food chemistry
Volume number 54
Issue number 26
Start page 9752
End page 9758
Publisher American Chemical Society
Place of publication Columbus, Ohio
Publication date 2006-12-27
ISSN 0021-8561
1520-5118
Keyword(s) carotenoids
Thraustochytrium sp. ONC-T18
antioxidants
astaxanthin
astaxanthin
canthaxanthin
echinenone
β-carotene
ultrasonic
co-enzyme Q10
Summary A variety of techniques for extracting carotenoids from the marine Thraustochytrium sp. ONC-T18 was compared. Specifically, the organic solvents acetone, ethyl acetate, and petroleum ether were tested, along with direct and indirect ultrasonic assisted extraction (probe vs bath) methods. Techniques that used petroleum ether/acetone/water (15:75:10, v/v/v) with 3 h of agitation, or 5 min in an ultrasonic bath, produced the highest extraction yields of total carotenoids (29−30.5 μg g-1). Concentrations up to 11.5 μg g-1 of canthaxanthin and 17.5 μg g-1 of β-carotene were detected in extracts stored for 6 weeks. Astaxanthin and echinenone were also detected as minor compounds. Extracts with and without antioxidants showed similar carotenoid concentration profiles. However, total carotenoid concentrations were approximately 8% higher when antioxidants were used. Finally, an easy-to-perform and inexpensive method to detect co-enzymes in ONC-T18 was also developed using silica gel TLC plates. Five percent methanol in toluene as a mobile phase consistently eluted co-enzyme Q10 standards and could separate the co-enzyme fractions present in ONC-T18.
Language eng
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006, American Chemical Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019492

Document type: Journal Article
Collection: School of Life and Environmental Sciences
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 14 times in TR Web of Science
Scopus Citation Count Cited 14 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 410 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Wed, 16 Sep 2009, 10:22:58 EST by Rachael Mackenzie

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.