Evaluation of fatty acid extraction methods for Thraustochytrium sp. ONC-T18

Burja, Adam M., Armenta, Roberto E., Radianingtyas, Helia and Barrow, Colin J. 2007, Evaluation of fatty acid extraction methods for Thraustochytrium sp. ONC-T18, Journal of agricultural and food chemistry, vol. 55, no. 12, pp. 4795-4801.

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Title Evaluation of fatty acid extraction methods for Thraustochytrium sp. ONC-T18
Formatted title Evaluation of fatty acid extraction methods for Thraustochytrium sp. ONC-T18
Author(s) Burja, Adam M.
Armenta, Roberto E.
Radianingtyas, Helia
Barrow, Colin J.
Journal name Journal of agricultural and food chemistry
Volume number 55
Issue number 12
Start page 4795
End page 4801
Publisher American Chemical Society
Place of publication Columbus, Ohio
Publication date 2007-06-13
ISSN 0021-8561
1520-5118
Keyword(s) Docosahexaenoic acid
eicosapentaenoic acid
polyunsaturated fatty acids
ω-3 fatty acids
Thraustochytrium sp. ONC-T18
lipids
extractions
ultrasonics
Summary Various extraction methods were assessed in their capacity to extract fatty acids from a dried biomass of Thraustochytrium sp. ONC-T18. Direct saponification using KOH in ethanol or in hexane:ethanol was one of the most efficient techniques to extract lipids (697 mg g-1). The highest amount of fatty acids (714 mg g-1) was extracted using a miniaturized Bligh and Dyer extraction technique. The use of ultrasonics to break down cell walls while extracting with solvents (methanol:chloroform) also offered high extraction yields of fatty acids (609 mg g-1). Moreover, when the transesterification mixture used for a direct transesterification method was doubled, the extraction of fatty acids increased approximately 77% (from 392 to 696 mg g-1). This work showed that Thraustochytrium sp. ONC-T18 has the ability to produce over 700 mg g-1 of lipids, including more than 165 mg g-1 of docosahexaenoic acid, which makes this microorganism a potential candidate for the commercial production of polyunsaturated fatty acids. Finally, other lipids, such as myristic, palmitic, palmitoleic, and oleic acids, were also produced and recovered in significant amounts (54, 196, 123, and 81 mg g-1), respectively.
Language eng
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2007, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019503

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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