Stimulation of cytokine production in human peripheral blood mononuclear cells by an aqueous Chlorella extract

Ewart, H. Stephen, Bloch, Olga, Girouard, Gabrielle S., Kralovec, Jaroslav, Barrow, Colin J., Ben-Yehudah, Gilad, Suarez, Erick Reyes and Rapoport, Micha J. 2007, Stimulation of cytokine production in human peripheral blood mononuclear cells by an aqueous Chlorella extract, Planta med, vol. 73, no. 8, pp. 762-768.

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Title Stimulation of cytokine production in human peripheral blood mononuclear cells by an aqueous Chlorella extract
Formatted title Stimulation of cytokine production in human peripheral blood mononuclear cells by an aqueous Chlorella extract
Author(s) Ewart, H. Stephen
Bloch, Olga
Girouard, Gabrielle S.
Kralovec, Jaroslav
Barrow, Colin J.
Ben-Yehudah, Gilad
Suarez, Erick Reyes
Rapoport, Micha J.
Journal name Planta med
Volume number 73
Issue number 8
Start page 762
End page 768
Publisher Georg Thieme Verlag
Place of publication Stuttgart, Germany
Publication date 2007
ISSN 0032-0943
1439-0221
Keyword(s) Chlorella pyrenoidosa
Chlorellaceae
immunostimulation
human peripheral blood mononuclear cells
T-helper-1 cells
T-helper-2 cells
Summary CPE is an aqueous extract of the edible micro alga Chlorella pyrenoidosa, which has been shown to have immunostimulatory effects in vivo. In the present study, CPE was evaluated for an ability to stimulate cytokine production by human peripheral blood mononuclear cells (PBMC). PBMC from healthy individuals were treated ex vivo for 24 hours with 1, 10 and 100 μg/mL CPE. This resulted in a marked increase in the level of IL-10, a regulatory cytokine, and strong stimulation of the T-helper-1 (Th1) cell cytokines, IFN-γ and TNF-α. In contrast, stimulation of representative T-helper-2 (Th2) cell cytokines, IL-4 and IL-13, was minor. CPE (1, 10 or 100 μg/mL) did not cause a proliferation of human PBMC suggesting that enhanced secretion of cytokines was not secondary to an increase in cell number. We conclude that CPE stimulation of human PBMC induces a Th1-patterned cytokine response and a strong anti-inflammatory regulatory cytokine response, observations that await confirmation in vivo.
Language eng
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2007, Georg Thieme Verlag KG
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019536

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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