Effects of conjugated linoleic acid on myogenic and inflammatory responses in a human primary muscle and tumor coculture model

Larsen, Amy and Crowe, Timothy 2009, Effects of conjugated linoleic acid on myogenic and inflammatory responses in a human primary muscle and tumor coculture model, Nutrition and cancer, vol. 61, no. 5, pp. 687-695.

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Title Effects of conjugated linoleic acid on myogenic and inflammatory responses in a human primary muscle and tumor coculture model
Author(s) Larsen, Amy
Crowe, Timothy
Journal name Nutrition and cancer
Volume number 61
Issue number 5
Start page 687
End page 695
Total pages 9
Publisher Routledge
Place of publication Abingdon, England
Publication date 2009-09
ISSN 0163-5581
1532-7914
Summary The antiproliferative and anti-inflammatory properties of conjugated linoleic acid (CLA) make it a potentially novel treatment in chronic inflammatory muscle wasting disease, particularly cancer cachexia. Human primary muscle cells were grown in coculture with MIA PaCa-2 pancreatic tumor cells and exposed to varying concentrations of c9,t11 and t10,c12 CLA. Expression of myogenic (Myf5, MyoD, myogenin, and myostatin) and inflammatory genes (CCL-2, COX-2, IL-8, and TNF-) were measured by real-time PCR. The t10,c12 CLA isomer, but not the c9,t11 isomer, significantly decreased MIA PaCa-2 proliferation by between 15% and 19%. There was a marked decrease in muscle MyoD and myogenin expression (78% and 62%, respectively), but no change in either Myf5 or myostatin, in myotubes grown in coculture with MIA PaCa-2 cells. CLA had limited influence on these responses. A similar pattern of myogenic gene expression changes was observed in myotubes treated with TNF- alone. Several-fold significant increases in CCL-2, COX-2, IL-8, and TNF- expression in myotubes were observed with MIA PaCa-2 coculture. The c9,t11 CLA isomer significantly decreased basal expression of TNF- in myotubes and could ameliorate its tumor-induced rise. The study provides insight into the anti-inflammatory and antiproliferative actions of CLA and its application as a therapeutic agent in inflammatory disease states.
Language eng
Field of Research 111204 Cancer Therapy (excl Chemotherapy and Radiation Therapy)
Socio Economic Objective 920102 Cancer and Related Disorders
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
HERDC collection year 2009
Copyright notice ©2009, Taylor & Francis Group
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019551

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